Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   U8327_RS08105 Genome accession   NZ_CP141765
Coordinates   1663070..1664503 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain HYM1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1658070..1669503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U8327_RS08075 (U8327_08075) yrbG 1658628..1659605 (+) 978 WP_000922873.1 calcium/sodium antiporter -
  U8327_RS08080 (U8327_08080) kdsD 1659619..1660605 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  U8327_RS08085 (U8327_08085) kdsC 1660626..1661192 (+) 567 WP_000030006.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  U8327_RS08090 (U8327_08090) lptC 1661189..1661764 (+) 576 WP_000030536.1 LPS export ABC transporter periplasmic protein LptC -
  U8327_RS08095 (U8327_08095) lptA 1661733..1662290 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  U8327_RS08100 (U8327_08100) lptB 1662297..1663022 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  U8327_RS08105 (U8327_08105) rpoN/rpoN1 1663070..1664503 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  U8327_RS08110 (U8327_08110) hpf 1664526..1664813 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  U8327_RS08115 (U8327_08115) ptsN 1664931..1665422 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  U8327_RS08120 (U8327_08120) rapZ 1665468..1666322 (+) 855 WP_000243741.1 RNase adapter RapZ -
  U8327_RS08125 (U8327_08125) npr 1666319..1666591 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  U8327_RS08130 (U8327_08130) yrbL 1666804..1667436 (+) 633 WP_000620396.1 PhoP regulatory network protein YrbL -
  U8327_RS08135 (U8327_08135) mtgA 1667433..1668161 (-) 729 WP_000047064.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  U8327_RS08140 (U8327_08140) elbB 1668158..1668811 (-) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=837543 U8327_RS08105 WP_000809051.1 1663070..1664503(+) (rpoN/rpoN1) [Escherichia coli strain HYM1]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=837543 U8327_RS08105 WP_000809051.1 1663070..1664503(+) (rpoN/rpoN1) [Escherichia coli strain HYM1]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGATACTGACCGCGCCATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGTTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATTATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACGGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCTTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCATTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465