Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   VA249_RS12965 Genome accession   NZ_AP024165
Coordinates   2813735..2814310 (-) Length   191 a.a.
NCBI ID   WP_221068219.1    Uniprot ID   -
Organism   Vibrio alfacsensis strain 04Ya108     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2808735..2819310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VA249_RS12940 (VYA_24300) aroB 2809075..2810175 (-) 1101 WP_221068216.1 3-dehydroquinate synthase -
  VA249_RS12945 (VYA_24310) aroK 2810204..2810722 (-) 519 WP_099080818.1 shikimate kinase AroK -
  VA249_RS12950 (VYA_24320) pilQ 2810911..2812590 (-) 1680 WP_420864276.1 type IV pilus secretin PilQ Machinery gene
  VA249_RS12955 (VYA_24330) pilP 2812650..2813159 (-) 510 WP_221068217.1 pilus assembly protein PilP Machinery gene
  VA249_RS12960 (VYA_24340) pilO 2813149..2813742 (-) 594 WP_221068218.1 type 4a pilus biogenesis protein PilO Machinery gene
  VA249_RS12965 (VYA_24350) pilN 2813735..2814310 (-) 576 WP_221068219.1 PilN domain-containing protein Machinery gene
  VA249_RS12970 (VYA_24360) pilM 2814294..2815307 (-) 1014 WP_221068220.1 type IV pilus biogenesis protein PilM Machinery gene
  VA249_RS12975 (VYA_24370) - 2815449..2818013 (+) 2565 WP_221068221.1 penicillin-binding protein 1A -
  VA249_RS12980 (VYA_24380) oxyR 2818085..2818993 (-) 909 WP_221068222.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22197.44 Da        Isoelectric Point: 10.2578

>NTDB_id=83744 VA249_RS12965 WP_221068219.1 2813735..2814310(-) (pilN) [Vibrio alfacsensis strain 04Ya108]
MLYRINLLPWREHQRKAHRHRFVSLAVFGVMIAAIIQWGGNQYLQAQQAEQQKRLDYLSNHITLFDQRIEAMKIAEQEHR
QVLQRLNVVDQLQRGRSKTTAFMNLIPSVIPEGVYVDKIKINHYYVELSGIADSTSHLATMLDNMERSAKLYEVDMHSIV
HGKSRFGRSFQQFNVSFRLREAQGAGSLHHG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=83744 VA249_RS12965 WP_221068219.1 2813735..2814310(-) (pilN) [Vibrio alfacsensis strain 04Ya108]
ATGTTGTATCGCATTAATTTGTTGCCATGGCGCGAGCATCAACGTAAGGCGCATCGTCACCGTTTTGTTAGCCTCGCTGT
TTTTGGCGTAATGATTGCGGCGATCATTCAGTGGGGAGGAAATCAGTATTTACAGGCTCAACAAGCTGAGCAGCAAAAGC
GTCTAGATTATCTCTCAAATCACATTACGCTTTTTGATCAGCGGATTGAAGCGATGAAAATTGCGGAGCAAGAGCATCGC
CAAGTTCTGCAGCGATTAAATGTTGTTGACCAATTACAGCGAGGACGAAGCAAAACGACGGCGTTTATGAATCTTATCCC
CTCGGTGATCCCTGAGGGGGTTTATGTCGATAAGATTAAAATCAATCACTATTACGTTGAGCTTTCTGGGATTGCAGATA
GCACATCCCATCTGGCGACGATGCTCGATAATATGGAGCGATCAGCAAAGCTTTATGAAGTAGATATGCATTCTATTGTT
CATGGAAAATCACGCTTTGGGAGATCATTCCAACAATTTAATGTCTCTTTTCGATTACGGGAAGCGCAAGGGGCAGGAAG
TCTACATCATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

64.398

100

0.644
  pilN Vibrio cholerae strain A1552

52.577

100

0.534

Multiple sequence alignment