Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   VA249_RS12970 Genome accession   NZ_AP024165
Coordinates   2814294..2815307 (-) Length   337 a.a.
NCBI ID   WP_221068220.1    Uniprot ID   -
Organism   Vibrio alfacsensis strain 04Ya108     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2809294..2820307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VA249_RS12945 (VYA_24310) aroK 2810204..2810722 (-) 519 WP_099080818.1 shikimate kinase AroK -
  VA249_RS12950 (VYA_24320) pilQ 2810911..2812590 (-) 1680 WP_420864276.1 type IV pilus secretin PilQ Machinery gene
  VA249_RS12955 (VYA_24330) pilP 2812650..2813159 (-) 510 WP_221068217.1 pilus assembly protein PilP Machinery gene
  VA249_RS12960 (VYA_24340) pilO 2813149..2813742 (-) 594 WP_221068218.1 type 4a pilus biogenesis protein PilO Machinery gene
  VA249_RS12965 (VYA_24350) pilN 2813735..2814310 (-) 576 WP_221068219.1 PilN domain-containing protein Machinery gene
  VA249_RS12970 (VYA_24360) pilM 2814294..2815307 (-) 1014 WP_221068220.1 type IV pilus biogenesis protein PilM Machinery gene
  VA249_RS12975 (VYA_24370) - 2815449..2818013 (+) 2565 WP_221068221.1 penicillin-binding protein 1A -
  VA249_RS12980 (VYA_24380) oxyR 2818085..2818993 (-) 909 WP_221068222.1 DNA-binding transcriptional regulator OxyR -
  VA249_RS12985 (VYA_24390) - 2819167..2819895 (+) 729 WP_128810014.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 337 a.a.        Molecular weight: 37664.06 Da        Isoelectric Point: 7.0389

>NTDB_id=83745 VA249_RS12970 WP_221068220.1 2814294..2815307(-) (pilM) [Vibrio alfacsensis strain 04Ya108]
MDKLVVTGIDIGHNSLKAVVLKPSGRHYAVLDYKEIILKECIVAENNTINHQEIVKTLKEIKKALPFGAKRVAISVPENA
VISKKLQIDPGLDSSERDYAVMQVFAHQSPFPLQELSLDFVLDDVKNKTDGLEHYQVFATRKDVVDSRVDALQQSGLKPV
LVEVASQSLGHMWKLATEHFPEKSTYCLLNVGNVKSSFTMFTEQGELFHKEFSSSSWSEKTPSVYQKGACLQVDESHQEQ
ALIERVQRQLQLYASFNGSLNITGIWITGDGFVTPQLKRALSSQLSMECETLNPFELFTLKASLHKHKAVQSHQFTAAAG
LAIRGIHWLEHGNVVSH

Nucleotide


Download         Length: 1014 bp        

>NTDB_id=83745 VA249_RS12970 WP_221068220.1 2814294..2815307(-) (pilM) [Vibrio alfacsensis strain 04Ya108]
ATGGACAAACTCGTCGTTACTGGCATCGACATAGGCCATAACAGTCTCAAAGCCGTGGTACTTAAGCCTAGTGGTCGCCA
TTACGCGGTACTGGACTACAAAGAAATAATATTGAAAGAATGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACACTTAAAGAGATAAAAAAAGCACTGCCTTTCGGGGCTAAACGTGTCGCGATTTCGGTGCCAGAAAATGCC
GTGATCAGCAAAAAACTGCAAATAGATCCAGGGTTAGATAGCAGTGAACGAGACTATGCGGTCATGCAAGTTTTCGCTCA
TCAATCACCTTTCCCCTTACAAGAGCTTAGTTTAGATTTTGTTTTAGATGATGTGAAGAATAAAACGGATGGACTTGAAC
ATTATCAAGTATTTGCTACTCGTAAGGACGTTGTTGATTCTCGAGTCGATGCTTTACAACAATCAGGGTTAAAGCCTGTG
TTAGTAGAGGTGGCATCACAAAGCTTAGGTCATATGTGGAAGCTCGCGACGGAGCATTTTCCAGAGAAAAGTACCTACTG
CTTGCTCAATGTCGGCAACGTTAAATCATCGTTCACAATGTTTACTGAACAGGGTGAATTATTTCACAAAGAGTTCTCAT
CAAGTTCATGGTCTGAAAAAACGCCATCAGTATATCAGAAAGGTGCTTGTTTACAGGTGGATGAATCACACCAAGAGCAA
GCTCTGATTGAGCGAGTTCAGCGTCAACTGCAGCTTTATGCTTCTTTCAATGGTTCTCTCAATATTACCGGTATCTGGAT
TACAGGTGACGGTTTTGTCACACCCCAATTAAAGAGAGCGTTATCGAGTCAATTGAGCATGGAATGTGAAACACTCAACC
CGTTCGAGTTGTTTACATTGAAGGCGTCACTGCATAAGCATAAGGCTGTTCAATCGCACCAGTTTACTGCTGCTGCGGGC
CTCGCGATTCGAGGTATTCATTGGTTGGAGCATGGCAATGTTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-211)

Type IV pilus assembly protein PilM; (PilM_2)

(240-325)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

63.235

100

0.638
  pilM Vibrio cholerae strain A1552

41.765

100

0.421

Multiple sequence alignment