Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   QN061_RS14025 Genome accession   NZ_CP126305
Coordinates   2987382..2987972 (-) Length   196 a.a.
NCBI ID   WP_020329445.1    Uniprot ID   S7I501
Organism   Vibrio fluvialis strain SD21SC3889PM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2982382..2992972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QN061_RS14005 (QN061_14005) aroB 2983300..2984385 (-) 1086 WP_020329448.1 3-dehydroquinate synthase -
  QN061_RS14010 (QN061_14010) aroK 2984413..2984931 (-) 519 WP_004728569.1 shikimate kinase AroK -
  QN061_RS14015 (QN061_14015) pilQ 2985119..2986855 (-) 1737 WP_038127863.1 type IV pilus secretin PilQ family protein Machinery gene
  QN061_RS14020 (QN061_14020) pilP 2986880..2987392 (-) 513 WP_020329446.1 pilus assembly protein PilP Machinery gene
  QN061_RS14025 (QN061_14025) pilO 2987382..2987972 (-) 591 WP_020329445.1 type 4a pilus biogenesis protein PilO Machinery gene
  QN061_RS14030 (QN061_14030) pilN 2987965..2988555 (-) 591 WP_020329444.1 PilN domain-containing protein Machinery gene
  QN061_RS14035 (QN061_14035) pilM 2988545..2989549 (-) 1005 WP_222741034.1 type IV pilus assembly protein PilM Machinery gene
  QN061_RS14040 (QN061_14040) - 2989692..2992172 (+) 2481 WP_020329441.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22720.13 Da        Isoelectric Point: 4.7023

>NTDB_id=837439 QN061_RS14025 WP_020329445.1 2987382..2987972(-) (pilO) [Vibrio fluvialis strain SD21SC3889PM]
MANLQELELDEIAEWPLLPQLAVLLLLVLALQGAGYWVYMLPKQDALEGLKQQEQTLKSTIRIKASKVATLPKLKIQLDE
LAERYDFLLRQLPVQKELASMLASVNELGLENTLTFTRIDWGEKQNQEFLYRLPLNIELTGNYHNIGDFSEAIARLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKPEVSDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=837439 QN061_RS14025 WP_020329445.1 2987382..2987972(-) (pilO) [Vibrio fluvialis strain SD21SC3889PM]
ATGGCTAACTTGCAGGAACTGGAACTGGATGAAATCGCTGAATGGCCTTTGTTGCCTCAGCTGGCCGTGTTGTTACTGCT
GGTTCTGGCATTGCAAGGTGCGGGCTATTGGGTATATATGTTGCCCAAGCAGGATGCGCTCGAAGGTTTAAAACAGCAGG
AACAAACGCTCAAATCGACGATACGTATCAAAGCCAGCAAAGTGGCCACGTTACCCAAACTCAAAATCCAACTGGATGAA
CTGGCAGAACGGTATGACTTCCTGCTGCGTCAACTGCCGGTGCAAAAAGAGCTTGCCAGTATGCTGGCGTCGGTTAACGA
GCTGGGGCTGGAAAATACGCTGACGTTCACGCGGATTGACTGGGGGGAGAAGCAGAATCAGGAATTCCTCTATCGTCTGC
CACTCAATATCGAACTGACTGGCAATTATCACAACATCGGCGACTTTTCCGAAGCGATTGCGCGCTTGCCACGCATCATC
AACTTTGACGATGTCGATTGGCAGCGCGTCAGTCAGGAGAGCAGCACGCTGCACTTTCGCGTCCGCGCTTACACCTATCA
GTTCAAACCGGAGGTGAGCGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S7I501

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

79.592

100

0.796
  pilO Vibrio campbellii strain DS40M4

68.947

96.939

0.668