Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OH686_RS00760 Genome accession   NZ_CP126126
Coordinates   151762..152967 (+) Length   401 a.a.
NCBI ID   WP_289535732.1    Uniprot ID   -
Organism   Pseudomonas sp. SO81     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 146762..157967
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OH686_RS00735 (OH686_00745) - 146856..147362 (+) 507 WP_289535727.1 DUF1993 domain-containing protein -
  OH686_RS00740 (OH686_00750) - 147420..149006 (-) 1587 WP_289535729.1 ABC-F family ATPase -
  OH686_RS00745 (OH686_00755) sph 149124..150635 (-) 1512 WP_289535731.1 sphingomyelin phosphodiesterase -
  OH686_RS00750 (OH686_00760) - 150883..151335 (+) 453 WP_289538757.1 YqaA family protein -
  OH686_RS00755 (OH686_00765) - 151338..151616 (-) 279 WP_021700093.1 peptidylprolyl isomerase -
  OH686_RS00760 (OH686_00770) pilU 151762..152967 (+) 1206 WP_289535732.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OH686_RS00765 (OH686_00775) - 153025..153921 (-) 897 WP_289535734.1 dienelactone hydrolase family protein -
  OH686_RS00770 (OH686_00785) - 154310..155176 (+) 867 WP_289535735.1 AraC family transcriptional regulator -
  OH686_RS00775 (OH686_00790) - 155287..155670 (-) 384 WP_289535736.1 hypothetical protein -
  OH686_RS23210 (OH686_00795) - 155779..156123 (-) 345 WP_354005275.1 carboxypeptidase-like regulatory domain-containing protein -
  OH686_RS00780 - 156223..156645 (-) 423 WP_289535737.1 hypothetical protein -
  OH686_RS00785 (OH686_00800) - 156736..157359 (-) 624 WP_289535739.1 hypothetical protein -
  OH686_RS00790 (OH686_00805) - 157462..157827 (-) 366 WP_289535741.1 hypothetical protein -

Sequence

Protein


Download         Length: 401 a.a.        Molecular weight: 44253.61 Da        Isoelectric Point: 5.9269

>NTDB_id=836755 OH686_RS00760 WP_289535732.1 151762..152967(+) (pilU) [Pseudomonas sp. SO81]
MDLTQMLKILSSQDGSDLYLSTGAPPCAKFNGVLKPLSQEPLKPGDVAAIAAGVMDELQRADFEKELEMNLAISVPNVGR
FRINIFKQRNEVSIVARNIKMEIPKFEDLKLPEVLLKTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTVDGKRRAAVEVLLGTPTIRDQIKRGDFSEIKETMEKSKNLGMQTFDQAL
IDLVHDGSIDEEEAVKNADSANNVRLKLKLYKDNPAAPQAAAPVPTPEAAPVAAPANWAMELKLEDIEEEAPPEDPGRQG
L

Nucleotide


Download         Length: 1206 bp        

>NTDB_id=836755 OH686_RS00760 WP_289535732.1 151762..152967(+) (pilU) [Pseudomonas sp. SO81]
ATGGACCTCACTCAGATGCTGAAAATCCTGTCCAGCCAGGACGGATCCGACCTCTACCTGTCCACCGGGGCGCCGCCCTG
CGCCAAGTTCAACGGGGTGCTCAAGCCGCTCAGCCAGGAGCCGCTCAAGCCGGGAGACGTGGCCGCCATCGCGGCCGGGG
TGATGGATGAGCTGCAGCGCGCCGATTTCGAGAAGGAGCTGGAGATGAACCTGGCCATCTCCGTGCCCAACGTCGGTCGC
TTCCGCATCAACATCTTCAAGCAGCGCAACGAGGTCTCCATCGTCGCGCGCAACATCAAGATGGAGATTCCCAAGTTCGA
GGACCTCAAGCTGCCCGAGGTGCTGCTCAAGACGGTGATGGAGAAGCGCGGCCTGGTGCTGTTCGTCGGTGGCACCGGCT
CGGGCAAGTCCACCTCCCTGGCGGCACTGATCGACTACCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAAGAC
CCGGTGGAGTACGTGCACCGGCACAAGAAATCGATCATCAACCAGCGCGAAGTGGGCGTGGACACCCGCAGCTTCCACGC
CGCGCTGAAGAACACCCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCATG
CCCTGGCCTTCGCCGACACCGGCCACCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAAGAGCGCAGGCCTCAGCTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
TCTGGTCAAGACGGTCGACGGCAAGCGCCGCGCCGCCGTTGAGGTGCTGCTCGGTACGCCGACCATTCGCGACCAGATCA
AGCGCGGCGATTTCTCCGAGATCAAGGAGACCATGGAGAAATCGAAGAACCTCGGCATGCAGACCTTCGACCAGGCACTG
ATCGACCTGGTCCACGACGGCAGCATCGACGAGGAGGAGGCAGTGAAGAACGCCGACTCGGCGAACAACGTGCGCCTCAA
GCTCAAGTTGTACAAGGACAACCCGGCCGCGCCGCAGGCTGCTGCGCCAGTCCCTACTCCCGAGGCCGCACCGGTCGCAG
CTCCGGCCAACTGGGCAATGGAGCTAAAACTGGAGGATATCGAGGAAGAGGCTCCGCCAGAGGATCCGGGGCGCCAGGGC
CTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.695

87.531

0.496
  pilU Vibrio cholerae strain A1552

52.857

87.282

0.461
  pilU Acinetobacter baylyi ADP1

51.705

87.781

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.683

94.264

0.374