Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   QMK53_RS15820 Genome accession   NZ_CP125379
Coordinates   3613713..3614777 (-) Length   354 a.a.
NCBI ID   WP_320329512.1    Uniprot ID   -
Organism   Pseudomonas sp. P8_139     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3608713..3619777
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK53_RS15795 (QMK53_15790) aroK 3609394..3609912 (-) 519 WP_024011270.1 shikimate kinase AroK -
  QMK53_RS15800 (QMK53_15795) pilQ 3609917..3611989 (-) 2073 WP_102355614.1 type IV pilus secretin PilQ Machinery gene
  QMK53_RS15805 (QMK53_15800) - 3612003..3612530 (-) 528 WP_320329514.1 pilus assembly protein PilP -
  QMK53_RS15810 (QMK53_15805) pilO 3612527..3613150 (-) 624 WP_102355616.1 type 4a pilus biogenesis protein PilO -
  QMK53_RS15815 (QMK53_15810) - 3613147..3613713 (-) 567 WP_320329513.1 PilN domain-containing protein -
  QMK53_RS15820 (QMK53_15815) pilM 3613713..3614777 (-) 1065 WP_320329512.1 pilus assembly protein PilM Machinery gene
  QMK53_RS15825 (QMK53_15820) - 3614997..3617441 (+) 2445 WP_371859052.1 penicillin-binding protein 1A -
  QMK53_RS15830 (QMK53_15825) - 3617708..3618976 (-) 1269 WP_102355620.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38204.78 Da        Isoelectric Point: 4.8983

>NTDB_id=831645 QMK53_RS15820 WP_320329512.1 3613713..3614777(-) (pilM) [Pseudomonas sp. P8_139]
MLGLFNKKTNMLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPPNAVVEKNIAELEGVGHTLSRVLVKARTGLRSVAV
AVAGSAVITKVIEMDAGLTDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFRDALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FSNMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=831645 QMK53_RS15820 WP_320329512.1 3613713..3614777(-) (pilM) [Pseudomonas sp. P8_139]
GTGCTGGGACTCTTCAATAAAAAGACCAATATGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGTTGGAGCT
GAGCCGCCAGGGCGAGCGCTATCGGGTCGAGGCCTATGCGGTGGAACCGCTGCCGCCCAATGCCGTGGTCGAAAAGAACA
TCGCCGAACTCGAAGGTGTGGGTCACACCTTGTCCCGGGTGCTGGTCAAGGCGCGCACCGGGCTCAGGAGCGTGGCGGTG
GCAGTGGCCGGTTCTGCGGTGATCACCAAGGTCATCGAAATGGACGCCGGGCTGACCGACGACGAGCTGGAAAACCAGCT
GAAGATCGAAGCCGACCAGTACATTCCCTACCCGCTGGACGAAGTCGCCATCGATTTCGAAGTTCAGGGTGTGTCGCCGC
GCAACCCGGAACGGGTCAACGTGTTGCTCGCCGCCTGCCGCAAGGAAAACGTCGAAGTCCGCGAAGCGGCGCTGGCGCTG
GCGGGGCTGACTGCGCGGGTGGTCGATGTCGAGGCCTACGCACTGGAGCGCTCATTCGGTCTGCTAGCCACCCAGTTGGC
GGCCTCGCAGGAGCGCCTGACGGTCGCGGTGGTGGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACAACGGCC
GCATCATCTATACCCGCGAGCAACTGTTCGGTGGTCGGCAACTGACCGAAGAGATCCAGCGTCGCTATGGCCTGACCGTC
GAGCAGGCCGGACTGGCGAAAAAGCAGGGTGGCCTGCCCGACGACTATGTCAGCGAAGTCTTGCAGCCGTTCCGTGATGC
GCTGGTGCAGCAGGTGTCGCGTTCATTGCAGTTTTTCTTTGCCTCCGGGCAGTACAACGCCGTCGATCACATCCTTTTGG
CCGGTGGCACGGCGTCGGTGCCAGGGTTGGATCGCCTGATCGAGCAACGGCTGGGCACTCCGACTCAAGTCGCCAATCCG
TTTTCCAATATGGCCTTGAGCAGCAAGGTCAACGCGGGGGCCCTGGCCAGTGATGCGCCGGCCTTGATGATCGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.588

100

0.486