Detailed information NTG0830676

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QLX61_RS02100
• Genome accession: NZ_CP125288
• Coordinates: 455655..456803 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain SF416
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 450655..461803

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QLX61_RS02075 (QLX61_02075)	-	451806..452102 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
QLX61_RS02080 (QLX61_02080)	-	452099..452692 (-)	594	WP_003084544.1	YggT family protein	-
QLX61_RS02085 (QLX61_02085)	proC	452703..453524 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
QLX61_RS02090 (QLX61_02090)	-	453536..454228 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
QLX61_RS02095 (QLX61_02095)	pilT	454443..455477 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
QLX61_RS02100 (QLX61_02100)	pilU	455655..456803 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
QLX61_RS02105 (QLX61_02105)	czcD	456811..457710 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
QLX61_RS02110 (QLX61_02110)	-	457865..458269 (+)	405	WP_003084560.1	NINE protein	-
QLX61_RS02115 (QLX61_02115)	-	458512..459885 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
QLX61_RS02120 (QLX61_02120)	-	459882..461066 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=830676 QLX61_RS02100 WP_003100959.1 455655..456803(+) (pilU) [Pseudomonas aeruginosa strain SF416]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=830676 QLX61_RS02100 WP_003100959.1 455655..456803(+) (pilU) [Pseudomonas aeruginosa strain SF416]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382