NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	QLX50_RS12035	Genome accession	NZ_CP125283
Coordinates	2493670..2494284 (-)	Length	204 a.a.
NCBI ID	WP_044802529.1	Uniprot ID	-
Organism	Bacillus velezensis strain IFST-221
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2488670..2499284

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QLX50_RS12005 (QLX50_12005)	rpsT	2488973..2489239 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
QLX50_RS12010 (QLX50_12010)	holA	2489256..2490297 (-)	1042	Protein_2318	DNA polymerase III subunit delta	-
QLX50_RS12015 (QLX50_12015)	-	2490337..2490488 (-)	152	Protein_2319	hypothetical protein	-
QLX50_RS12020 (QLX50_12020)	-	2490529..2490663 (+)	135	WP_003152870.1	YqzM family protein	-
QLX50_RS12025 (QLX50_12025)	comEC	2490682..2493033 (-)	2352	WP_044802530.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
QLX50_RS12030 (QLX50_12030)	-	2493034..2493603 (-)	570	WP_003152868.1	ComE operon protein 2	-
QLX50_RS12035 (QLX50_12035)	comEA	2493670..2494284 (-)	615	WP_044802529.1	helix-hairpin-helix domain-containing protein	Machinery gene
QLX50_RS12040 (QLX50_12040)	comER	2494343..2495164 (+)	822	WP_012118027.1	late competence protein ComER	-
QLX50_RS12045 (QLX50_12045)	-	2495233..2495970 (-)	738	WP_282999704.1	class I SAM-dependent methyltransferase	-
QLX50_RS12050 (QLX50_12050)	rsfS	2495967..2496323 (-)	357	WP_007408260.1	ribosome silencing factor	-
QLX50_RS12055 (QLX50_12055)	yqeK	2496341..2496901 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
QLX50_RS12060 (QLX50_12060)	-	2496891..2497460 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
QLX50_RS12065 (QLX50_12065)	yhbY	2497471..2497761 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
QLX50_RS12070 (QLX50_12070)	aroE	2497755..2498597 (-)	843	WP_044802528.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22238.49 Da Isoelectric Point: 7.7754

                                >NTDB_id=830626 QLX50_RS12035 WP_044802529.1 2493670..2494284(-) (comEA) [Bacillus velezensis strain IFST-221]

MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELKVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKESAAVNINTASLDELQAISGVG

QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=830626 QLX50_RS12035 WP_044802529.1 2493670..2494284(-) (comEA) [Bacillus velezensis strain IFST-221]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT

TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAAAGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGACCGAAAACAG

CAGCGGGTGAAAAGGAGAGTGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.639	93.627	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0830626