Detailed information NTG0830350

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QMA76_RS14415
• Genome accession: NZ_CP125223
• Coordinates: 2985019..2986137 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain EGA65
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2980019..2991137

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QMA76_RS14405 (QMA76_14405)	-	2983135..2983827 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
QMA76_RS14410 (QMA76_14410)	pilT	2983954..2984991 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
QMA76_RS14415 (QMA76_14415)	pilU	2985019..2986137 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QMA76_RS14420 (QMA76_14420)	fur	2986207..2986644 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
QMA76_RS14425 (QMA76_14425)	bamE	2986756..2987154 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
QMA76_RS14430 (QMA76_14430)	-	2987209..2987532 (-)	324	WP_000442587.1	RnfH family protein	-
QMA76_RS14435 (QMA76_14435)	-	2987532..2988599 (-)	1068	WP_002019572.1	hypothetical protein	-
QMA76_RS14440 (QMA76_14440)	-	2988752..2989207 (-)	456	WP_000782976.1	bacteriohemerythrin	-
QMA76_RS14445 (QMA76_14445)	-	2989460..2990308 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=830350 QMA76_RS14415 WP_000347039.1 2985019..2986137(+) (pilU) [Acinetobacter baumannii strain EGA65]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=830350 QMA76_RS14415 WP_000347039.1 2985019..2986137(+) (pilU) [Acinetobacter baumannii strain EGA65]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395