Detailed information NTG0830221

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: P4G16_RS20250
• Genome accession: NZ_CP125166
• Coordinates: 4142083..4142883 (+)
• Length: 266 a.a.
• NCBI ID: WP_004191960.1
• Uniprot ID: A0AAJ5B1F9
• Organism: Klebsiella pneumoniae strain 161
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4137083..4147883

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P4G16_RS20215 (P4G16_20215)	gspH	4137239..4137754 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
P4G16_RS20220 (P4G16_20220)	gspI	4137751..4138116 (+)	366	WP_044241933.1	type II secretion system minor pseudopilin GspI	-
P4G16_RS20225 (P4G16_20225)	gspJ	4138113..4138709 (+)	597	WP_019705881.1	type II secretion system minor pseudopilin GspJ	-
P4G16_RS20230 (P4G16_20230)	gspK	4138706..4139692 (+)	987	WP_004183041.1	type II secretion system minor pseudopilin GspK	-
P4G16_RS20235 (P4G16_20235)	gspL	4139679..4140872 (+)	1194	WP_073558002.1	type II secretion system protein GspL	-
P4G16_RS20240 (P4G16_20240)	-	4140865..4141350 (+)	486	WP_004145896.1	type II secretion system protein M	-
P4G16_RS20245 (P4G16_20245)	-	4141340..4142086 (+)	747	WP_023316460.1	type II secretion system protein N	-
P4G16_RS20250 (P4G16_20250)	pilD	4142083..4142883 (+)	801	WP_004191960.1	A24 family peptidase	Machinery gene
P4G16_RS20255 (P4G16_20255)	hrpB	4142931..4145360 (-)	2430	WP_031588872.1	ATP-dependent helicase HrpB	-
P4G16_RS20260 (P4G16_20260)	thpR	4145433..4145969 (+)	537	WP_032416682.1	RNA 2',3'-cyclic phosphodiesterase	-
P4G16_RS20265 (P4G16_20265)	sfsA	4145969..4146685 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
P4G16_RS20270 (P4G16_20270)	dksA	4146848..4147303 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29403.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=830221 P4G16_RS20250 WP_004191960.1 4142083..4142883(+) (pilD) [Klebsiella pneumoniae strain 161]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=830221 P4G16_RS20250 WP_004191960.1 4142083..4142883(+) (pilD) [Klebsiella pneumoniae strain 161]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGTGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACTCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421