Detailed information NTG0082821

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: H7676_RS00675
• Genome accession: NZ_AP023390
• Coordinates: 104555..104989 (+)
• Length: 144 a.a.
• NCBI ID: WP_020904833.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain KUN-0014944
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 99555..109989

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H7676_RS00645 (KUN4944_00790)	-	101209..101574 (+)	366	WP_002986560.1	DUF1033 family protein	-
H7676_RS00650 (KUN4944_00800)	comYA	101667..102620 (+)	954	WP_184492312.1	competence type IV pilus ATPase ComGA	Machinery gene
H7676_RS00655 (KUN4944_00810)	comYB	102556..103590 (+)	1035	WP_226316548.1	competence type IV pilus assembly protein ComGB	Machinery gene
H7676_RS00660 (KUN4944_00820)	comYC	103592..103918 (+)	327	WP_002986552.1	competence type IV pilus major pilin ComGC	Machinery gene
H7676_RS00665 (KUN4944_00830)	comGD	103893..104321 (+)	429	WP_002986548.1	competence type IV pilus minor pilin ComGD	-
H7676_RS00670 (KUN4944_00840)	comGE	104278..104562 (+)	285	WP_011284422.1	competence type IV pilus minor pilin ComGE	-
H7676_RS00675 (KUN4944_00850)	comYF	104555..104989 (+)	435	WP_020904833.1	competence type IV pilus minor pilin ComGF	Machinery gene
H7676_RS00680 (KUN4944_00860)	comGG	104973..105299 (+)	327	WP_002986539.1	competence type IV pilus minor pilin ComGG	-
H7676_RS00685 (KUN4944_00870)	comYH	105397..106350 (+)	954	WP_010921802.1	class I SAM-dependent methyltransferase	Machinery gene
H7676_RS00690 (KUN4944_00880)	-	106409..107605 (+)	1197	WP_020904834.1	acetate kinase	-
H7676_RS00695	-	107792..108100 (+)	309	Protein_90	hypothetical protein	-
H7676_RS00700 (KUN4944_00890)	proC	108183..108953 (-)	771	WP_002986527.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16672.29 Da
• Isoelectric Point: 10.2526

>NTDB_id=82821 H7676_RS00675 WP_020904833.1 104555..104989(+) (comYF) [Streptococcus pyogenes strain KUN-0014944]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRNHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide

• Length: 435 bp

>NTDB_id=82821 H7676_RS00675 WP_020904833.1 104555..104989(+) (comYF) [Streptococcus pyogenes strain KUN-0014944]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACTCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAATCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	50.36	96.528	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	46.715	95.139	0.444
comGF/cglF	Streptococcus mitis NCTC 12261	44.776	93.056	0.417
comGF/cglF	Streptococcus pneumoniae Rx1	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae D39	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae R6	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae TIGR4	43.609	92.361	0.403
comGF/cglF	Streptococcus mitis SK321	42.857	92.361	0.396