Detailed information NTG0827540

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QJ951_RS02010
• Genome accession: NZ_CP124668
• Coordinates: 428755..429903 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain 2021CK-01445
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 423755..434903

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QJ951_RS01985 (QJ951_01985)	-	424906..425202 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
QJ951_RS01990 (QJ951_01990)	-	425199..425792 (-)	594	WP_003084544.1	YggT family protein	-
QJ951_RS01995 (QJ951_01995)	proC	425803..426624 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
QJ951_RS02000 (QJ951_02000)	-	426636..427328 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
QJ951_RS02005 (QJ951_02005)	pilT	427543..428577 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
QJ951_RS02010 (QJ951_02010)	pilU	428755..429903 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
QJ951_RS02015 (QJ951_02015)	czcD	429911..430810 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
QJ951_RS02020 (QJ951_02020)	-	430965..431369 (+)	405	WP_003084560.1	NINE protein	-
QJ951_RS02025 (QJ951_02025)	-	431612..432985 (+)	1374	WP_023098360.1	pyridoxal-phosphate dependent enzyme	-
QJ951_RS02030 (QJ951_02030)	-	432982..434166 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=827540 QJ951_RS02010 WP_003110016.1 428755..429903(+) (pilU) [Pseudomonas aeruginosa strain 2021CK-01445]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=827540 QJ951_RS02010 WP_003110016.1 428755..429903(+) (pilU) [Pseudomonas aeruginosa strain 2021CK-01445]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382