Detailed information NTG0827470

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QJ983_RS02130
• Genome accession: NZ_CP124666
• Coordinates: 451198..452346 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain 2021CK-01283
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 446198..457346

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QJ983_RS02105 (QJ983_02105)	-	447349..447645 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
QJ983_RS02110 (QJ983_02110)	-	447642..448235 (-)	594	WP_003084544.1	YggT family protein	-
QJ983_RS02115 (QJ983_02115)	proC	448246..449067 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
QJ983_RS02120 (QJ983_02120)	-	449079..449771 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
QJ983_RS02125 (QJ983_02125)	pilT	449986..451020 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
QJ983_RS02130 (QJ983_02130)	pilU	451198..452346 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
QJ983_RS02135 (QJ983_02135)	czcD	452354..453253 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
QJ983_RS02140 (QJ983_02140)	-	453408..453812 (+)	405	WP_003084560.1	NINE protein	-
QJ983_RS02145 (QJ983_02145)	-	454055..455428 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
QJ983_RS02150 (QJ983_02150)	-	455425..456609 (+)	1185	WP_031686325.1	PLP-dependent aspartate aminotransferase family protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=827470 QJ983_RS02130 WP_003110016.1 451198..452346(+) (pilU) [Pseudomonas aeruginosa strain 2021CK-01283]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=827470 QJ983_RS02130 WP_003110016.1 451198..452346(+) (pilU) [Pseudomonas aeruginosa strain 2021CK-01283]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCTGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382