Detailed information NTG0827402

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QJ960_RS01970
• Genome accession: NZ_CP124664
• Coordinates: 422579..423727 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 2021CK-01256
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 417579..428727

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QJ960_RS01945 (QJ960_01945)	-	418730..419026 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
QJ960_RS01950 (QJ960_01950)	-	419023..419616 (-)	594	WP_003084544.1	YggT family protein	-
QJ960_RS01955 (QJ960_01955)	proC	419627..420448 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
QJ960_RS01960 (QJ960_01960)	-	420460..421152 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
QJ960_RS01965 (QJ960_01965)	pilT	421367..422401 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
QJ960_RS01970 (QJ960_01970)	pilU	422579..423727 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
QJ960_RS01975 (QJ960_01975)	czcD	423735..424634 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
QJ960_RS01980 (QJ960_01980)	-	424788..425192 (+)	405	WP_003084560.1	NINE protein	-
QJ960_RS01985 (QJ960_01985)	-	425435..426808 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
QJ960_RS01990 (QJ960_01990)	-	426805..427989 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=827402 QJ960_RS01970 WP_003100959.1 422579..423727(+) (pilU) [Pseudomonas aeruginosa strain 2021CK-01256]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=827402 QJ960_RS01970 WP_003100959.1 422579..423727(+) (pilU) [Pseudomonas aeruginosa strain 2021CK-01256]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382