Detailed information NTG0827303

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P7I96_RS02250
• Genome accession: NZ_CP124660
• Coordinates: 489622..490770 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 2022CK-00160
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 484622..495770

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P7I96_RS02225 (P7I96_02225)	-	485773..486069 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
P7I96_RS02230 (P7I96_02230)	-	486066..486659 (-)	594	WP_003084544.1	YggT family protein	-
P7I96_RS02235 (P7I96_02235)	proC	486670..487491 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
P7I96_RS02240 (P7I96_02240)	-	487503..488195 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
P7I96_RS02245 (P7I96_02245)	pilT	488410..489444 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
P7I96_RS02250 (P7I96_02250)	pilU	489622..490770 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
P7I96_RS02255 (P7I96_02255)	czcD	490778..491677 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
P7I96_RS02260 (P7I96_02260)	-	491831..492235 (+)	405	WP_003084560.1	NINE protein	-
P7I96_RS02265 (P7I96_02265)	-	492478..493851 (+)	1374	WP_015502220.1	pyridoxal-phosphate dependent enzyme	-
P7I96_RS02270 (P7I96_02270)	-	493848..495032 (+)	1185	WP_023086800.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=827303 P7I96_RS02250 WP_003100959.1 489622..490770(+) (pilU) [Pseudomonas aeruginosa strain 2022CK-00160]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=827303 P7I96_RS02250 WP_003100959.1 489622..490770(+) (pilU) [Pseudomonas aeruginosa strain 2022CK-00160]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382