Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   P7I93_RS29865 Genome accession   NZ_CP124658
Coordinates   6282736..6283788 (-) Length   350 a.a.
NCBI ID   WP_034055307.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2022CK-00068     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 6283893..6285197 6282736..6283788 flank 105


Gene organization within MGE regions


Location: 6282736..6285197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7I93_RS29865 (P7I93_29865) pilM 6282736..6283788 (-) 1053 WP_034055307.1 type IV pilus assembly protein PilM Machinery gene
  P7I93_RS29870 (P7I93_29870) - 6283893..6285197 (+) 1305 WP_010793081.1 IS1380-like element ISPa33 family transposase -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37586.95 Da        Isoelectric Point: 4.5405

>NTDB_id=827297 P7I93_RS29865 WP_034055307.1 6282736..6283788(-) (pilM) [Pseudomonas aeruginosa strain 2022CK-00068]
MKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVVAVAG
SAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALALAGLT
AKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSVEEAG
LAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANPFADM
ALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=827297 P7I93_RS29865 WP_034055307.1 6282736..6283788(-) (pilM) [Pseudomonas aeruginosa strain 2022CK-00068]
ATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTTGAGCCGCTCCGG
AGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACATCGTCGAGCTGG
AAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTCGCCGTCGCCGGT
TCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCTGAAGATCGAGGC
CGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGCGCAATCCCGAGC
GCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTGGCCGGCCTGACC
GCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGGTGCCGACACCGA
CCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGACGCACCATCTATA
CCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTGGAGGAAGCCGGT
CTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGCCGTGGTGCAGCA
GGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGGCGGGCGGTACGG
CGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCGTTCGCCGACATG
GCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGGCCTGGCGTTGAG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(10-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.84

100

0.56
  comM Acinetobacter nosocomialis M2

55.84

100

0.56
  comM Acinetobacter baylyi ADP1

51.862

99.714

0.517
  pilM Legionella pneumophila strain ERS1305867

46.286

100

0.463