Detailed information NTG0827269

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P7I93_RS02080
• Genome accession: NZ_CP124658
• Coordinates: 438578..439726 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain 2022CK-00068
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 433578..444726

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P7I93_RS02055 (P7I93_02055)	-	434729..435025 (-)	297	WP_023980267.1	DUF167 domain-containing protein	-
P7I93_RS02060 (P7I93_02060)	-	435022..435615 (-)	594	WP_003084544.1	YggT family protein	-
P7I93_RS02065 (P7I93_02065)	proC	435626..436447 (-)	822	WP_023092309.1	pyrroline-5-carboxylate reductase	-
P7I93_RS02070 (P7I93_02070)	-	436459..437151 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
P7I93_RS02075 (P7I93_02075)	pilT	437366..438400 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
P7I93_RS02080 (P7I93_02080)	pilU	438578..439726 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
P7I93_RS02085 (P7I93_02085)	czcD	439734..440633 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
P7I93_RS02090 (P7I93_02090)	-	440788..441192 (+)	405	WP_003084560.1	NINE protein	-
P7I93_RS02095 (P7I93_02095)	-	441435..442808 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
P7I93_RS02100 (P7I93_02100)	-	442805..443989 (+)	1185	WP_023092310.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=827269 P7I93_RS02080 WP_003110016.1 438578..439726(+) (pilU) [Pseudomonas aeruginosa strain 2022CK-00068]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=827269 P7I93_RS02080 WP_003110016.1 438578..439726(+) (pilU) [Pseudomonas aeruginosa strain 2022CK-00068]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382