Detailed information NTG0827067

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P7I80_RS02295
• Genome accession: NZ_CP124649
• Coordinates: 513749..514897 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain 2020CK-00218
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 508749..519897

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P7I80_RS02270 (P7I80_02270)	-	509900..510196 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
P7I80_RS02275 (P7I80_02275)	-	510193..510786 (-)	594	WP_003084544.1	YggT family protein	-
P7I80_RS02280 (P7I80_02280)	proC	510797..511618 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
P7I80_RS02285 (P7I80_02285)	-	511630..512322 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
P7I80_RS02290 (P7I80_02290)	pilT	512537..513571 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
P7I80_RS02295 (P7I80_02295)	pilU	513749..514897 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
P7I80_RS02300 (P7I80_02300)	czcD	514905..515804 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
P7I80_RS02305 (P7I80_02305)	-	515959..516363 (+)	405	WP_003084560.1	NINE protein	-
P7I80_RS02310 (P7I80_02310)	-	516606..517979 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
P7I80_RS02315 (P7I80_02315)	-	517976..519160 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=827067 P7I80_RS02295 WP_003110016.1 513749..514897(+) (pilU) [Pseudomonas aeruginosa strain 2020CK-00218]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=827067 P7I80_RS02295 WP_003110016.1 513749..514897(+) (pilU) [Pseudomonas aeruginosa strain 2020CK-00218]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382