Detailed information NTG0827049

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P7I81_RS13860
• Genome accession: NZ_CP124648
• Coordinates: 2945797..2946945 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain 2020CK-00220
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2940797..2951945

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P7I81_RS13835 (P7I81_13835)	-	2941948..2942244 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
P7I81_RS13840 (P7I81_13840)	-	2942241..2942834 (-)	594	WP_003084544.1	YggT family protein	-
P7I81_RS13845 (P7I81_13845)	proC	2942845..2943666 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
P7I81_RS13850 (P7I81_13850)	-	2943678..2944370 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
P7I81_RS13855 (P7I81_13855)	pilT	2944585..2945619 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
P7I81_RS13860 (P7I81_13860)	pilU	2945797..2946945 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
P7I81_RS13865 (P7I81_13865)	czcD	2946953..2947852 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
P7I81_RS13870 (P7I81_13870)	-	2948006..2948410 (+)	405	WP_003084560.1	NINE protein	-
P7I81_RS13875 (P7I81_13875)	-	2948653..2950026 (+)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
P7I81_RS13880 (P7I81_13880)	-	2950023..2951207 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=827049 P7I81_RS13860 WP_003110016.1 2945797..2946945(+) (pilU) [Pseudomonas aeruginosa strain 2020CK-00220]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=827049 P7I81_RS13860 WP_003110016.1 2945797..2946945(+) (pilU) [Pseudomonas aeruginosa strain 2020CK-00220]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382