Detailed information NTG0827001

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P7I78_RS02110
• Genome accession: NZ_CP124646
• Coordinates: 454262..455410 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain 2020CK-00185
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 449262..460410

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P7I78_RS02085 (P7I78_02085)	-	450413..450709 (-)	297	WP_031632852.1	DUF167 domain-containing protein	-
P7I78_RS02090 (P7I78_02090)	-	450706..451299 (-)	594	WP_003084544.1	YggT family protein	-
P7I78_RS02095 (P7I78_02095)	proC	451310..452131 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
P7I78_RS02100 (P7I78_02100)	-	452143..452835 (-)	693	WP_004365038.1	YggS family pyridoxal phosphate-dependent enzyme	-
P7I78_RS02105 (P7I78_02105)	pilT	453050..454084 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
P7I78_RS02110 (P7I78_02110)	pilU	454262..455410 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
P7I78_RS02115 (P7I78_02115)	czcD	455418..456317 (-)	900	WP_004365039.1	CDF family zinc transporter CzcD	-
P7I78_RS02120 (P7I78_02120)	-	456472..456876 (+)	405	WP_003084560.1	NINE protein	-
P7I78_RS02125 (P7I78_02125)	-	457119..458492 (+)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
P7I78_RS02130 (P7I78_02130)	-	458489..459673 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=827001 P7I78_RS02110 WP_003110016.1 454262..455410(+) (pilU) [Pseudomonas aeruginosa strain 2020CK-00185]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=827001 P7I78_RS02110 WP_003110016.1 454262..455410(+) (pilU) [Pseudomonas aeruginosa strain 2020CK-00185]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTATATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382