Detailed information NTG0826870

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P7I86_RS01990
• Genome accession: NZ_CP124626
• Coordinates: 420546..421694 (-)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain 2021CK-01161
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 415546..426694

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P7I86_RS01970 (P7I86_01970)	-	416283..417467 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
P7I86_RS01975 (P7I86_01975)	-	417464..418837 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
P7I86_RS01980 (P7I86_01980)	-	419080..419484 (-)	405	WP_003084560.1	NINE protein	-
P7I86_RS01985 (P7I86_01985)	czcD	419639..420538 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
P7I86_RS01990 (P7I86_01990)	pilU	420546..421694 (-)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
P7I86_RS01995 (P7I86_01995)	pilT	421872..422906 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
P7I86_RS02000 (P7I86_02000)	-	423121..423813 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
P7I86_RS02005 (P7I86_02005)	proC	423825..424646 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
P7I86_RS02010 (P7I86_02010)	-	424657..425250 (+)	594	WP_003084544.1	YggT family protein	-
P7I86_RS02015 (P7I86_02015)	-	425247..425543 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=826870 P7I86_RS01990 WP_003110016.1 420546..421694(-) (pilU) [Pseudomonas aeruginosa strain 2021CK-01161]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=826870 P7I86_RS01990 WP_003110016.1 420546..421694(-) (pilU) [Pseudomonas aeruginosa strain 2021CK-01161]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382