Detailed information NTG0823813

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: R6I15_RS26500
• Genome accession: NZ_CP137716
• Coordinates: 5727756..5728820 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: A0A0H2ZID6
• Organism: Pseudomonas aeruginosa isolate FELIX_MS463
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5722756..5733820

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R6I15_RS26455 (R6I15_26455)	-	5723144..5723758 (+)	615	WP_019485954.1	hypothetical protein	-
R6I15_RS26460 (R6I15_26460)	-	5723751..5723951 (+)	201	WP_019485953.1	hypothetical protein	-
R6I15_RS26465 (R6I15_26465)	-	5723944..5724567 (+)	624	WP_015649401.1	DUF3164 family protein	-
R6I15_RS26470 (R6I15_26470)	-	5724569..5724865 (+)	297	WP_019485952.1	hypothetical protein	-
R6I15_RS26475 (R6I15_26475)	-	5724852..5725418 (+)	567	WP_019485951.1	regulatory protein GemA	-
R6I15_RS26480 (R6I15_26480)	-	5725624..5725992 (+)	369	WP_019485949.1	Mor transcription activator family protein	-
R6I15_RS26485 (R6I15_26485)	pilP	5726097..5726543 (-)	447	Protein_5229	type 4a pilus biogenesis lipoprotein PilP	-
R6I15_RS26490 (R6I15_26490)	pilO	5726540..5727163 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	Machinery gene
R6I15_RS26495 (R6I15_26495)	pilN	5727160..5727756 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	Machinery gene
R6I15_RS26500 (R6I15_26500)	pilM	5727756..5728820 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
R6I15_RS26505 (R6I15_26505)	-	5729005..5731473 (+)	2469	WP_003114576.1	penicillin-binding protein 1A	-
R6I15_RS26510 (R6I15_26510)	-	5731578..5732846 (-)	1269	WP_003103278.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=823813 R6I15_RS26500 WP_003110888.1 5727756..5728820(-) (pilM) [Pseudomonas aeruginosa isolate FELIX_MS463]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=823813 R6I15_RS26500 WP_003110888.1 5727756..5728820(-) (pilM) [Pseudomonas aeruginosa isolate FELIX_MS463]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAAAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0H2ZID6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM/pilM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466