Detailed information NTG0823665

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QE150_RS14270
• Genome accession: NZ_CP123993
• Coordinates: 2861235..2862353 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain Ab4294
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2856235..2867353

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QE150_RS14260 (QE150_14265)	-	2859350..2860042 (-)	693	WP_270901149.1	YggS family pyridoxal phosphate-dependent enzyme	-
QE150_RS14265 (QE150_14270)	pilT	2860169..2861206 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
QE150_RS14270 (QE150_14275)	pilU	2861235..2862353 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QE150_RS14275 (QE150_14280)	fur	2862424..2862861 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
QE150_RS14280 (QE150_14285)	bamE	2862973..2863371 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
QE150_RS14285 (QE150_14290)	-	2863426..2863749 (-)	324	WP_000442584.1	RnfH family protein	-
QE150_RS14290 (QE150_14295)	-	2863749..2864816 (-)	1068	WP_002084046.1	hypothetical protein	-
QE150_RS14295 (QE150_14300)	-	2864969..2865424 (-)	456	WP_000782976.1	bacteriohemerythrin	-
QE150_RS14300 (QE150_14305)	-	2865677..2866525 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=823665 QE150_RS14270 WP_000347036.1 2861235..2862353(+) (pilU) [Acinetobacter baumannii strain Ab4294]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=823665 QE150_RS14270 WP_000347036.1 2861235..2862353(+) (pilU) [Acinetobacter baumannii strain Ab4294]
ATGGATTTTAATGACTTGCTTAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGAATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTCATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392