Detailed information NTG0823265

Bioinformatically predicted

Overview

• Name: pilC
• Type: Machinery gene
• Locus tag: QH638_RS10760
• Genome accession: NZ_CP123958
• Coordinates: 2090454..2091650 (+)
• Length: 398 a.a.
• NCBI ID: WP_006248403.1
• Uniprot ID: A0A249A0I8
• Organism: Mannheimia haemolytica strain 190176
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2085454..2096650

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QH638_RS10730	proB	2085850..2086934 (-)	1085	Protein_2066	glutamate 5-kinase	-
QH638_RS10735	folA	2087046..2087540 (+)	495	WP_006248397.1	type 3 dihydrofolate reductase	-
QH638_RS10740	-	2087660..2087890 (+)	231	WP_006248398.1	YgjV family protein	-
QH638_RS10745	radA	2087945..2089324 (-)	1380	WP_006248399.1	DNA repair protein RadA	-
QH638_RS10750	pilA	2089505..2089984 (+)	480	WP_006248401.1	prepilin peptidase-dependent pilin	Machinery gene
QH638_RS10755	-	2090005..2090448 (+)	444	Protein_2071	pilus assembly protein PilB	-
QH638_RS10760	pilC	2090454..2091650 (+)	1197	WP_006248403.1	type II secretion system F family protein	Machinery gene
QH638_RS10765	-	2091653..2092309 (+)	657	WP_006248404.1	prepilin peptidase	-
QH638_RS10770	coaE	2092345..2092980 (+)	636	WP_006248405.1	dephospho-CoA kinase	-
QH638_RS10775	yacG	2092962..2093147 (+)	186	WP_006248406.1	DNA gyrase inhibitor YacG	-
QH638_RS10780	holA	2093148..2094173 (-)	1026	WP_006253426.1	DNA polymerase III subunit delta	-
QH638_RS10785	lptE	2094216..2094704 (-)	489	WP_006248408.1	LPS assembly lipoprotein LptE	-

Sequence

Protein

• Length: 398 a.a.
• Molecular weight: 45186.75 Da
• Isoelectric Point: 9.4698

>NTDB_id=823265 QH638_RS10760 WP_006248403.1 2090454..2091650(+) (pilC) [Mannheimia haemolytica strain 190176]
MTKIYEFHWQAQSRFGQKQRGKRLATNRDMLENLLLAQGYQHIRISRNFVLSANPKKEEVNSLLSQLALLVNSAMPLKQA
LSMLLQNCRNIRLYLWLNELIQHIESGYAFSQSLEKLGEFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKLEKLAKKVK
KILFYPVIVLLISLALSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGTVLLGTLLLAVIFYLFFAKKSRL
VAKWKMALWAKMPLFSRIIEQSRIIFFSQNIALMLNAHIPLDVALRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGIN
PNVFGAEMAQMLEVGEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFDMGALVE

Nucleotide

• Length: 1197 bp

>NTDB_id=823265 QH638_RS10760 WP_006248403.1 2090454..2091650(+) (pilC) [Mannheimia haemolytica strain 190176]
ATGACGAAAATCTATGAATTTCATTGGCAAGCCCAGAGCCGATTTGGGCAAAAACAGCGGGGCAAGCGGTTGGCAACGAA
TCGAGACATGTTGGAAAACCTTTTGCTGGCACAAGGTTATCAGCATATTCGGATTAGCCGAAATTTTGTGCTTTCAGCAA
ACCCGAAAAAAGAAGAGGTGAATAGCTTGCTATCACAGCTTGCATTGTTGGTTAATTCTGCCATGCCGTTAAAACAGGCA
TTATCTATGTTGTTGCAAAACTGCCGAAATATCCGCCTTTATCTATGGCTTAATGAGCTGATTCAGCATATTGAAAGTGG
CTATGCTTTTTCACAATCGTTGGAGAAATTGGGTGAATTTGTGACCAATCAGGAAATTCAGCTAATCAAAATGGGCGAAC
AAAGCGGAAAACTGGGCATTATGCTGACTAATTTAGCTGAAAGCCGAGCGAAATTGGAAAAATTGGCGAAAAAAGTGAAG
AAAATCCTGTTTTACCCGGTGATTGTGTTGCTGATTTCGCTCGCTTTATCTATCGGGCTATTGGTTTTGATTGTACCGCA
GTTTGCCCAACTTTATGGCTCAAAAGACAAAACTTTACCCCTCATTACCGATATTTTATTTACCCTTTCCGAATGCTTGA
TTGAGCAAGGCACAGTATTACTTGGCACGTTACTACTAGCGGTCATTTTTTACCTGTTTTTTGCAAAAAAGAGCAGGTTA
GTCGCAAAGTGGAAAATGGCTCTATGGGCTAAAATGCCACTGTTTAGCCGAATTATTGAGCAATCTCGCATTATCTTTTT
CAGCCAAAATATTGCCTTAATGCTGAATGCTCATATTCCGCTTGATGTTGCCTTGAGGTCGTTTTTATCCGAGAAAAATG
ATGACCCGATTTTACAGCAAGAAATTGGCACTATGCTTAATTTGTTACAACAAGGCTACAAATTTTCACAAGGGATCAAC
CCGAATGTGTTTGGTGCTGAGATGGCACAAATGCTTGAGGTTGGGGAGAAAAGTGGCAATGTGGCGAAAATGTGTGAGCA
TATTAGTGAGATGTACCAACAAAAATTAGATTACCAAATTGATATTTTGTCTCAGCTATTAGAACCGATGCTAATGTTGA
TAATGGGCGTGATTGTCGGCACTATTATTGTTGGGCTGTATTTACCGATTTTTGATATGGGGGCTTTGGTGGAGTAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (64-186)

• Type II secretion system (T2SS), protein F (T2SSF) (267-389)

3D structure

• AlphaFold DB: A0A249A0I8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilC	Glaesserella parasuis strain SC1401	54	100	0.543
pilC	Haemophilus influenzae Rd KW20	42.145	100	0.425
pilC	Haemophilus influenzae 86-028NP	41.147	100	0.415