Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QH638_RS10750 Genome accession   NZ_CP123958
Coordinates   2089505..2089984 (+) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 190176     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2084505..2094984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QH638_RS10725 - 2085086..2085850 (-) 765 WP_006248395.1 TSUP family transporter -
  QH638_RS10730 proB 2085850..2086934 (-) 1085 Protein_2066 glutamate 5-kinase -
  QH638_RS10735 folA 2087046..2087540 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  QH638_RS10740 - 2087660..2087890 (+) 231 WP_006248398.1 YgjV family protein -
  QH638_RS10745 radA 2087945..2089324 (-) 1380 WP_006248399.1 DNA repair protein RadA -
  QH638_RS10750 pilA 2089505..2089984 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  QH638_RS10755 - 2090005..2090448 (+) 444 Protein_2071 pilus assembly protein PilB -
  QH638_RS10760 pilC 2090454..2091650 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  QH638_RS10765 - 2091653..2092309 (+) 657 WP_006248404.1 prepilin peptidase -
  QH638_RS10770 coaE 2092345..2092980 (+) 636 WP_006248405.1 dephospho-CoA kinase -
  QH638_RS10775 yacG 2092962..2093147 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  QH638_RS10780 holA 2093148..2094173 (-) 1026 WP_006253426.1 DNA polymerase III subunit delta -
  QH638_RS10785 lptE 2094216..2094704 (-) 489 WP_006248408.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=823264 QH638_RS10750 WP_006248401.1 2089505..2089984(+) (pilA) [Mannheimia haemolytica strain 190176]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=823264 QH638_RS10750 WP_006248401.1 2089505..2089984(+) (pilA) [Mannheimia haemolytica strain 190176]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491