Detailed information NTG0823184

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P4N66_RS02025
• Genome accession: NZ_CP123953
• Coordinates: 433211..434359 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 59
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 428211..439359

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P4N66_RS02000	-	429362..429658 (-)	297	WP_031628073.1	DUF167 domain-containing protein	-
P4N66_RS02005 (P4N66_gene0401)	-	429655..430248 (-)	594	WP_003084544.1	YggT family protein	-
P4N66_RS02010 (P4N66_gene0402)	proC	430259..431080 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
P4N66_RS02015 (P4N66_gene0403)	-	431092..431784 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
P4N66_RS02020 (P4N66_gene0404)	pilT	431999..433033 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
P4N66_RS02025 (P4N66_gene0405)	pilU	433211..434359 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
P4N66_RS02030 (P4N66_gene0406)	czcD	434367..435266 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
P4N66_RS02035 (P4N66_gene0407)	-	435421..435825 (+)	405	WP_003084560.1	NINE protein	-
P4N66_RS02040 (P4N66_gene0408)	-	436068..437441 (+)	1374	WP_015502220.1	pyridoxal-phosphate dependent enzyme	-
P4N66_RS02045 (P4N66_gene0409)	-	437438..438622 (+)	1185	WP_044061315.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=823184 P4N66_RS02025 WP_003100959.1 433211..434359(+) (pilU) [Pseudomonas aeruginosa strain 59]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=823184 P4N66_RS02025 WP_003100959.1 433211..434359(+) (pilU) [Pseudomonas aeruginosa strain 59]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATTACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGATTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382