Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   QAO71_RS14805 Genome accession   NZ_CP123445
Coordinates   3159199..3160263 (-) Length   354 a.a.
NCBI ID   WP_036989108.1    Uniprot ID   A0A031MG26
Organism   Halopseudomonas sp. SMJS2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3154199..3165263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QAO71_RS14780 (QAO71_14780) aroK 3154713..3155228 (-) 516 WP_280164770.1 shikimate kinase AroK -
  QAO71_RS14785 (QAO71_14785) pilQ 3155234..3157410 (-) 2177 Protein_2888 type IV pilus secretin PilQ -
  QAO71_RS14790 (QAO71_14790) pilP 3157468..3157998 (-) 531 WP_036989112.1 type 4a pilus biogenesis lipoprotein PilP -
  QAO71_RS14795 (QAO71_14795) - 3157995..3158624 (-) 630 WP_074777498.1 type 4a pilus biogenesis protein PilO -
  QAO71_RS14800 (QAO71_14800) - 3158621..3159199 (-) 579 WP_074777496.1 PilN domain-containing protein -
  QAO71_RS14805 (QAO71_14805) pilM 3159199..3160263 (-) 1065 WP_036989108.1 pilus assembly protein PilM Machinery gene
  QAO71_RS14810 (QAO71_14810) - 3160449..3162848 (+) 2400 WP_280164771.1 penicillin-binding protein 1A -
  QAO71_RS14815 (QAO71_14815) - 3163045..3164736 (+) 1692 WP_280164772.1 AMP-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38365.75 Da        Isoelectric Point: 4.2717

>NTDB_id=821145 QAO71_RS14805 WP_036989108.1 3159199..3160263(-) (pilM) [Halopseudomonas sp. SMJS2]
MLGLFKKKANTLLGIDISSTTVKLLELSRSGNRYKVEAYAVEPLPPGAVAEKNIIELEGVGQTLEKVLARSRSSLKQSAV
AVAGSAVITKLIEMDAGLDDDEMEEQIKLEADQYIPYPLDEVSIDFEVQGVSPRSPERVEVLLAACRRENVDIREAALAL
AGLDVKVVEVEAYAMERACELVVDQLDGNPDDLTIAVIDIGATMMTLSVLSQGRTIYTREQLFGGKQLTDEIQRRYGLSQ
EEAGRAKKQGGLPDDYESEVLEPFKDAVAQQVSRSLQFFFAGGQYQDVDYILLAGGTASIPGLDQLIQQKLGTTTLVANP
FANMTLSNKVNAVALSNDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=821145 QAO71_RS14805 WP_036989108.1 3159199..3160263(-) (pilM) [Halopseudomonas sp. SMJS2]
GTGCTAGGGCTATTCAAAAAAAAGGCGAATACGCTGCTGGGTATTGATATCAGTTCCACAACCGTGAAACTGCTTGAACT
CAGCCGATCAGGGAACCGCTACAAGGTGGAGGCCTATGCGGTTGAACCGCTCCCACCCGGCGCTGTAGCGGAAAAAAACA
TCATCGAACTCGAAGGCGTGGGCCAGACCCTGGAAAAGGTGCTGGCCCGTTCGCGTTCCTCGCTGAAACAGTCGGCTGTC
GCCGTGGCTGGCTCGGCGGTGATTACCAAACTCATCGAAATGGATGCCGGCCTCGATGACGACGAGATGGAAGAGCAGAT
CAAGCTTGAGGCGGATCAGTATATTCCGTACCCGCTTGACGAGGTCTCCATCGATTTCGAAGTGCAGGGCGTCTCGCCGC
GCAGCCCCGAACGGGTCGAGGTATTGCTGGCGGCCTGTCGTCGGGAGAACGTCGATATCCGCGAAGCCGCACTGGCGCTG
GCCGGGCTGGACGTCAAGGTCGTCGAGGTCGAGGCCTACGCCATGGAGCGGGCCTGCGAACTGGTAGTCGATCAGCTCGA
CGGCAATCCCGACGATCTGACCATTGCGGTGATCGACATCGGCGCCACCATGATGACCCTCAGCGTGCTGAGCCAGGGAC
GCACCATCTATACCCGGGAGCAGCTGTTCGGCGGCAAGCAACTGACCGACGAGATCCAGCGCCGTTATGGGCTGTCCCAG
GAAGAGGCCGGCCGGGCCAAGAAGCAGGGCGGCTTGCCGGACGACTACGAGAGCGAGGTGCTTGAGCCGTTCAAGGATGC
GGTGGCGCAGCAGGTGTCCCGCTCATTGCAGTTCTTCTTCGCCGGTGGTCAGTACCAGGATGTCGACTACATTCTGCTGG
CCGGCGGGACTGCCTCGATTCCGGGACTGGATCAACTGATCCAGCAGAAACTCGGCACCACCACTCTGGTGGCCAACCCC
TTTGCAAACATGACCCTGTCGAACAAGGTGAATGCGGTCGCGCTCAGCAATGACGCGCCGGCACTGATGATCGCCTGTGG
CCTGGCAATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A031MG26

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.65

100

0.557
  comM Acinetobacter nosocomialis M2

55.65

100

0.557
  comM Acinetobacter baylyi ADP1

53.107

100

0.531
  pilM Legionella pneumophila strain ERS1305867

47.175

100

0.472