Detailed information NTG0820841

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R5018_RS03540
• Genome accession: NZ_CP137491
• Coordinates: 761034..762182 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain HPA1406
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 756034..767182

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R5018_RS03515 (R5018_03515)	-	757185..757481 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
R5018_RS03520 (R5018_03520)	-	757478..758071 (-)	594	WP_003084544.1	YggT family protein	-
R5018_RS03525 (R5018_03525)	proC	758082..758903 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
R5018_RS03530 (R5018_03530)	-	758915..759607 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
R5018_RS03535 (R5018_03535)	pilT	759822..760856 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
R5018_RS03540 (R5018_03540)	pilU	761034..762182 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
R5018_RS03545 (R5018_03545)	czcD	762190..763089 (-)	900	WP_003084557.1	CDF family zinc transporter CzcD	-
R5018_RS03550 (R5018_03550)	-	763244..763648 (+)	405	WP_003084560.1	NINE protein	-
R5018_RS03555 (R5018_03555)	-	763891..765264 (+)	1374	WP_126892857.1	pyridoxal-phosphate dependent enzyme	-
R5018_RS03560 (R5018_03560)	-	765261..766445 (+)	1185	WP_003084564.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=820841 R5018_RS03540 WP_003100959.1 761034..762182(+) (pilU) [Pseudomonas aeruginosa strain HPA1406]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=820841 R5018_RS03540 WP_003100959.1 761034..762182(+) (pilU) [Pseudomonas aeruginosa strain HPA1406]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382