Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   R5019_RS28345 Genome accession   NZ_CP137490
Coordinates   6052736..6053272 (+) Length   178 a.a.
NCBI ID   WP_033945690.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain HPA2017     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6047736..6058272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5019_RS28320 (R5019_28320) yacG 6047905..6048105 (-) 201 WP_017148551.1 DNA gyrase inhibitor YacG -
  R5019_RS28325 (R5019_28325) coaE 6048102..6048713 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  R5019_RS28330 (R5019_28330) pilD 6048710..6049582 (-) 873 WP_017148550.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  R5019_RS28335 (R5019_28335) pilC 6049585..6050802 (-) 1218 WP_023103506.1 type II secretion system F family protein Machinery gene
  R5019_RS28340 (R5019_28340) pilB 6050806..6052509 (-) 1704 WP_023100230.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R5019_RS28345 (R5019_28345) pilE 6052736..6053272 (+) 537 WP_033945690.1 pilin Machinery gene
  R5019_RS28350 (R5019_28350) tfpZ 6053337..6054074 (+) 738 WP_023110409.1 TfpX/TfpZ family type IV pilin accessory protein -
  R5019_RS28360 (R5019_28360) nadC 6054308..6055156 (-) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R5019_RS28365 (R5019_28365) - 6055442..6057697 (+) 2256 WP_003104913.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 178 a.a.        Molecular weight: 18264.80 Da        Isoelectric Point: 6.2201

>NTDB_id=820816 R5019_RS28345 WP_033945690.1 6052736..6053272(+) (pilE) [Pseudomonas aeruginosa strain HPA2017]
MKAQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRTRVSEGLVLAEPAKLMISTDGSASTADLTRATTTWNQQSNNLGA
SSKYVTSVLMDAGNTGVITITYVAGQVGLPTAGNTLILSPYINDGNTRTALATAVAAGTRGTIDWACTSASNATATAQGF
TGMAAGSVPQEFAPAQCR

Nucleotide


Download         Length: 537 bp        

>NTDB_id=820816 R5019_RS28345 WP_033945690.1 6052736..6053272(+) (pilE) [Pseudomonas aeruginosa strain HPA2017]
ATGAAAGCTCAAAAAGGCTTCACTTTGATCGAGCTGATGATCGTTGTAGCGATCATCGGTATTCTGGCTGCGATTGCTCT
GCCGGCCTATCAGGACTACACCGTTCGTACCCGTGTCAGTGAAGGTCTGGTTCTGGCTGAGCCCGCCAAACTGATGATCT
CCACGGATGGAAGTGCTTCTACTGCTGATCTGACTCGCGCAACCACTACTTGGAACCAGCAATCCAATAATCTGGGTGCA
TCCAGTAAGTACGTAACATCTGTTTTGATGGATGCTGGTAACACCGGTGTTATCACTATTACGTATGTCGCTGGCCAAGT
GGGCTTGCCGACTGCCGGTAACACTCTGATTCTGTCTCCCTATATCAATGATGGTAACACTCGCACTGCTCTGGCGACTG
CAGTGGCTGCTGGCACTCGTGGCACCATTGATTGGGCTTGCACTTCGGCATCGAACGCGACTGCCACTGCTCAGGGTTTC
ACCGGCATGGCAGCTGGTAGTGTGCCGCAAGAGTTTGCTCCTGCTCAGTGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-178)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

43.719

100

0.489
  pilA Ralstonia pseudosolanacearum GMI1000

40.556

100

0.41
  pilA2 Legionella pneumophila strain ERS1305867

40.341

98.876

0.399
  pilA2 Legionella pneumophila str. Paris

39.773

98.876

0.393