Detailed information NTG0820659

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R5025_RS07090
• Genome accession: NZ_CP137487
• Coordinates: 1549980..1551128 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain HPA2120
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1544980..1556128

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R5025_RS07065 (R5025_07065)	-	1546131..1546427 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
R5025_RS07070 (R5025_07070)	-	1546424..1547017 (-)	594	WP_003084544.1	YggT family protein	-
R5025_RS07075 (R5025_07075)	proC	1547028..1547849 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
R5025_RS07080 (R5025_07080)	-	1547861..1548553 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
R5025_RS07085 (R5025_07085)	pilT	1548768..1549802 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
R5025_RS07090 (R5025_07090)	pilU	1549980..1551128 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
R5025_RS07095 (R5025_07095)	czcD	1551136..1552035 (-)	900	WP_023913256.1	CDF family zinc transporter CzcD	-
R5025_RS07100 (R5025_07100)	-	1552190..1552594 (+)	405	WP_003084560.1	NINE protein	-
R5025_RS07105 (R5025_07105)	-	1552837..1554210 (+)	1374	WP_023098360.1	pyridoxal-phosphate dependent enzyme	-
R5025_RS07110 (R5025_07110)	-	1554207..1555391 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=820659 R5025_RS07090 WP_003110016.1 1549980..1551128(+) (pilU) [Pseudomonas aeruginosa strain HPA2120]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=820659 R5025_RS07090 WP_003110016.1 1549980..1551128(+) (pilU) [Pseudomonas aeruginosa strain HPA2120]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382