Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   R4535_RS12165 Genome accession   NZ_CP137091
Coordinates   2692551..2694062 (-) Length   503 a.a.
NCBI ID   WP_000138174.1    Uniprot ID   P37093
Organism   Vibrio cholerae strain DL4211     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2687551..2699062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4535_RS12135 (R4535_12110) gspK 2688234..2689244 (-) 1011 WP_001201881.1 type II secretion system minor pseudopilin GspK -
  R4535_RS12140 (R4535_12115) gspJ 2689234..2689902 (-) 669 WP_001958988.1 type II secretion system minor pseudopilin GspJ -
  R4535_RS12145 (R4535_12120) gspI 2689889..2690242 (-) 354 WP_000837699.1 type II secretion system minor pseudopilin GspI -
  R4535_RS12150 (R4535_12125) gspH 2690232..2690816 (-) 585 WP_000127545.1 type II secretion system minor pseudopilin GspH -
  R4535_RS12155 (R4535_12130) gspG 2690850..2691290 (-) 441 WP_000738789.1 type II secretion system major pseudopilin GspG -
  R4535_RS12160 (R4535_12135) gspF 2691331..2692551 (-) 1221 WP_057555100.1 type II secretion system inner membrane protein GspF -
  R4535_RS12165 (R4535_12140) pilB/pilB1 2692551..2694062 (-) 1512 WP_000138174.1 type II secretion system ATPase GspE Machinery gene
  R4535_RS12170 (R4535_12145) gspD 2694059..2696086 (-) 2028 WP_000878555.1 type II secretion system secretin GspD -
  R4535_RS12175 (R4535_12150) gspC 2696132..2697049 (-) 918 WP_000394665.1 type II secretion system protein GspC -
  R4535_RS12180 (R4535_12155) hslR 2697279..2697656 (+) 378 WP_000098851.1 ribosome-associated heat shock protein Hsp15 -
  R4535_RS12185 (R4535_12160) hslO 2697690..2698565 (+) 876 WP_175597522.1 Hsp33 family molecular chaperone HslO -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 56358.58 Da        Isoelectric Point: 6.2107

>NTDB_id=818261 R4535_RS12165 WP_000138174.1 2692551..2694062(-) (pilB/pilB1) [Vibrio cholerae strain DL4211]
MTEMVISPAERQSIRRLPFSFANRFKLVLDWNEDFSQASIYYLAPLSMEALVETKRVVKHAFQLIELSQAEFESKLTQVY
QRDSSEARQLMEDIGADSDDFFSLAEELPQNEDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKTLSIRFRVDGV
LREVLAPSRKLSSLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLG
MTAHNHDNFRRLIKRPHGIILVTGPTGSGKSTTLYAGLQELNSSERNILTVEDPIEFDIDGIGQTQVNPRVDMTFARGLR
AILRQDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAVTRLRDMGIEPFLISSSLLGVLAQRLVRTLCPDC
KEPYEADKEQRKLFDSKKKEPLILYRATGCPKCNHKGYRGRTGIHELLLVDDALQELIHSEAGEQAMEKHIRATTPSIRD
DGLDKVRQGITSLEEVMRVTKES

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=818261 R4535_RS12165 WP_000138174.1 2692551..2694062(-) (pilB/pilB1) [Vibrio cholerae strain DL4211]
ATGACCGAAATGGTGATCTCTCCAGCTGAGCGACAGTCGATTCGTCGTCTGCCCTTTAGCTTCGCCAATCGCTTTAAGTT
GGTGCTGGATTGGAATGAGGATTTCTCCCAAGCCAGCATTTATTACTTAGCGCCGTTGTCGATGGAGGCGCTCGTCGAAA
CCAAGCGGGTGGTCAAGCACGCTTTCCAACTGATTGAGCTCTCTCAAGCGGAGTTTGAAAGCAAGCTAACCCAAGTCTAT
CAGCGTGATTCTTCAGAAGCGCGCCAATTGATGGAAGACATTGGTGCCGACAGTGATGACTTCTTCTCACTGGCTGAAGA
GCTGCCTCAAAACGAAGATTTGCTAGAAAGTGAAGATGATGCGCCGATCATCAAATTGATCAACGCCATGTTGGGCGAAG
CGATCAAAGAAGGCGCGTCAGATATTCACATTGAAACTTTTGAAAAAACACTGTCGATCCGTTTTCGAGTCGATGGCGTG
CTGCGCGAAGTACTGGCACCCAGCCGCAAACTCTCATCGCTGCTCGTTTCACGGGTCAAAGTGATGGCCAAGCTCGACAT
TGCCGAAAAACGTGTGCCGCAAGATGGCCGTATTTCGCTGCGTATCGGTGGCCGCGCGGTCGATGTGCGGGTTTCAACCA
TGCCTTCATCACATGGTGAGCGAGTGGTAATGCGTTTGCTGGATAAAAACGCGACGCGCCTTGATCTGCACAGTTTGGGT
ATGACGGCGCATAACCATGATAACTTCCGCCGTTTGATTAAGCGGCCGCATGGGATCATTCTGGTGACTGGTCCAACCGG
CTCGGGTAAATCGACCACCTTGTACGCGGGTTTGCAAGAGCTCAACAGCAGCGAGCGCAACATTTTAACCGTAGAAGATC
CGATCGAATTTGATATTGATGGTATCGGTCAGACCCAAGTCAACCCAAGGGTAGACATGACCTTCGCGCGGGGGCTACGC
GCTATTTTGCGTCAAGACCCGGATGTGGTGATGGTGGGGGAAATTCGTGACTTGGAAACGGCGCAAATTGCAGTGCAAGC
CTCGCTGACCGGTCACTTAGTGATGTCGACTCTGCACACCAACACGGCCGTTGGGGCGGTGACTCGGCTGCGCGATATGG
GCATCGAGCCTTTTTTGATCTCTTCTTCATTGCTCGGTGTTCTGGCTCAGCGCTTGGTGCGCACCTTATGCCCAGATTGC
AAAGAGCCTTACGAGGCGGACAAAGAGCAGCGCAAACTGTTTGATAGCAAGAAAAAAGAACCGCTGATCCTTTATCGCGC
CACGGGCTGCCCTAAATGTAACCACAAAGGTTACCGTGGCCGAACCGGTATCCACGAGCTGCTGCTGGTGGATGATGCGT
TGCAAGAGCTGATCCATAGCGAGGCGGGCGAACAGGCGATGGAGAAACACATTCGCGCGACCACGCCGAGCATTCGTGAT
GATGGTTTAGATAAGGTGCGCCAAGGCATTACTTCGCTAGAAGAAGTGATGCGGGTGACTAAGGAGTCCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(129-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 1P9R
  PDB 1P9W
  PDB 2BH1
  PDB 4KSR
  PDB 4KSS

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

41.412

100

0.431
  pilB Legionella pneumophila strain ERS1305867

47.126

86.481

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

44.619

88.668

0.396
  pilB Vibrio campbellii strain DS40M4

44.118

87.873

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.745

77.932

0.388
  pilB Vibrio cholerae strain A1552

48.485

78.728

0.382
  pilF Thermus thermophilus HB27

47.761

79.92

0.382
  pilB Acinetobacter baumannii D1279779

46.21

81.312

0.376