Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   LLUL033_RS11725 Genome accession   NZ_CP122980
Coordinates   2302009..2302434 (-) Length   141 a.a.
NCBI ID   WP_014735174.1    Uniprot ID   -
Organism   Lactococcus cremoris strain UL033     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2297145..2311108 2302009..2302434 within 0


Gene organization within MGE regions


Location: 2297145..2311108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLUL033_RS11700 (LLUL033_11705) - 2298611..2299420 (-) 810 WP_011677177.1 metal ABC transporter permease -
  LLUL033_RS11705 (LLUL033_11710) - 2299413..2300150 (-) 738 WP_332370438.1 metal ABC transporter ATP-binding protein -
  LLUL033_RS11710 (LLUL033_11715) - 2300329..2301171 (-) 843 WP_011836040.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  LLUL033_RS11715 (LLUL033_11720) - 2301168..2301605 (-) 438 WP_011836041.1 zinc-dependent MarR family transcriptional regulator -
  LLUL033_RS11720 (LLUL033_11725) comGG 2301686..2301985 (-) 300 WP_011836042.1 competence type IV pilus minor pilin ComGG Machinery gene
  LLUL033_RS11725 (LLUL033_11730) comGF 2302009..2302434 (-) 426 WP_014735174.1 competence type IV pilus minor pilin ComGF Machinery gene
  LLUL033_RS11730 (LLUL033_11735) comGE 2302418..2302714 (-) 297 WP_014735175.1 competence type IV pilus minor pilin ComGE Machinery gene
  LLUL033_RS11735 (LLUL033_11740) comGD 2302686..2303117 (-) 432 WP_260368600.1 competence type IV pilus minor pilin ComGD Machinery gene
  LLUL033_RS11740 (LLUL033_11745) comGC 2303077..2303454 (-) 378 WP_375794176.1 competence type IV pilus major pilin ComGC Machinery gene
  LLUL033_RS11745 (LLUL033_11750) comGB 2303472..2304497 (-) 1026 WP_042211510.1 competence type IV pilus assembly protein ComGB Machinery gene
  LLUL033_RS11750 (LLUL033_11755) comGA 2304397..2305377 (-) 981 WP_011836048.1 competence type IV pilus ATPase ComGA Machinery gene
  LLUL033_RS11755 (LLUL033_11760) - 2305491..2309273 (-) 3783 Protein_2290 PolC-type DNA polymerase III -
  LLUL033_RS11760 - 2309805..2310398 (-) 594 Protein_2291 PolC-type DNA polymerase III N-terminal domain-containing protein -
  LLUL033_RS11765 (LLUL033_11765) - 2310572..2311108 (-) 537 WP_011836050.1 hypothetical protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 16210.78 Da        Isoelectric Point: 8.4549

>NTDB_id=817693 LLUL033_RS11725 WP_014735174.1 2302009..2302434(-) (comGF) [Lactococcus cremoris strain UL033]
MKLKIRAFTLLECLVALLAISGSVLVISGLTRMIEEQMKISQNDSRKDWQIFCEQMRSELSGAKLDNVNQNFLYVTKDKK
LRFGLVGDDFRKSDDKGQGYQPMLYDLKGAKIQAEENLIKITIDFDNGGERVFIYRFTDTK

Nucleotide


Download         Length: 426 bp        

>NTDB_id=817693 LLUL033_RS11725 WP_014735174.1 2302009..2302434(-) (comGF) [Lactococcus cremoris strain UL033]
TTGAAGCTCAAAATTAGGGCATTTACTTTACTAGAATGTCTCGTAGCACTTTTGGCAATTTCTGGTTCAGTGCTAGTCAT
CTCAGGGCTGACTAGAATGATTGAAGAACAGATGAAAATAAGCCAGAATGATAGTAGGAAAGATTGGCAAATATTTTGTG
AACAAATGCGTTCAGAACTCTCCGGAGCAAAATTAGACAATGTGAATCAAAATTTTTTGTATGTGACCAAAGATAAGAAG
TTACGGTTCGGATTAGTAGGGGATGATTTTCGTAAAAGTGATGATAAAGGGCAAGGATACCAACCGATGCTTTATGATTT
AAAAGGAGCAAAAATTCAGGCAGAAGAAAATTTGATAAAAATAACAATTGATTTTGATAATGGAGGTGAGCGAGTATTTA
TTTATCGATTTACAGATACAAAGTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(38-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Lactococcus lactis subsp. cremoris KW2

100

100

1
  comYF Streptococcus mutans UA140

43.972

100

0.44
  comYF Streptococcus mutans UA159

43.262

100

0.433
  comGF/cglF Streptococcus mitis NCTC 12261

39.286

99.291

0.39
  comGF/cglF Streptococcus mitis SK321

39.286

99.291

0.39
  comGF/cglF Streptococcus pneumoniae Rx1

37.857

99.291

0.376
  comGF/cglF Streptococcus pneumoniae D39

37.857

99.291

0.376
  comGF/cglF Streptococcus pneumoniae R6

37.857

99.291

0.376
  comGF/cglF Streptococcus pneumoniae TIGR4

37.857

99.291

0.376