Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   R3J35_RS09325 Genome accession   NZ_CP136972
Coordinates   2009067..2010125 (-) Length   352 a.a.
NCBI ID   WP_004086598.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain CFBP8173     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2004067..2015125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R3J35_RS09305 (R3J35_09285) comQ/pilQ 2005301..2007211 (-) 1911 WP_038210668.1 type IV pilus secretin PilQ Machinery gene
  R3J35_RS09310 (R3J35_09290) - 2007211..2007740 (-) 530 Protein_1817 pilus assembly protein PilP -
  R3J35_RS09315 (R3J35_09295) - 2007737..2008405 (-) 669 WP_004086596.1 type 4a pilus biogenesis protein PilO -
  R3J35_RS09320 (R3J35_09300) - 2008515..2009067 (-) 553 Protein_1819 PilN domain-containing protein -
  R3J35_RS09325 (R3J35_09305) pilM 2009067..2010125 (-) 1059 WP_004086598.1 type IV pilus biogenesis protein PilM Machinery gene
  R3J35_RS09330 (R3J35_09310) - 2010365..2012794 (+) 2430 WP_021358378.1 penicillin-binding protein 1A -
  R3J35_RS09335 (R3J35_09315) - 2013098..2014063 (-) 966 WP_004086600.1 aldo/keto reductase -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37539.19 Da        Isoelectric Point: 4.6541

>NTDB_id=816959 R3J35_RS09325 WP_004086598.1 2009067..2010125(-) (pilM) [Xylella fastidiosa subsp. multiplex strain CFBP8173]
MGLFSKKQSVLVGVDISSTAVKLLQLSRSGNRFKVEHYAVEPLPLNAVAEKGIVEVEQVGEAIRRAVSRSGTKAKFAAAA
VAGSAVITKLIPMPAGLEEQDLEAQIEIEATNYIPYPIEEVSLDFEVLGPVPNNTEMVQVLLAASRSENVELRQSALELG
GLTAKVIDVEALAVENAFSLIAQELSVGSNALVALIDIGATMSTLNVLHSGRSLYAREQLFGGKQLTDEVMHRYGMTYEE
AGQAKRQGGLPQSYDVEVLGPFKDSVIQQISRLLQFFYAGSEYNRVDCIVLAGGCAVIAGLPAMVEERLGVVTVVANPLA
QMTLGAKVQAHTLAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=816959 R3J35_RS09325 WP_004086598.1 2009067..2010125(-) (pilM) [Xylella fastidiosa subsp. multiplex strain CFBP8173]
GTGGGGCTTTTTTCCAAAAAACAGTCGGTATTGGTTGGTGTCGACATTAGTTCAACTGCAGTGAAGCTCTTACAGCTTTC
CCGCAGTGGTAATCGCTTCAAGGTGGAACACTATGCTGTGGAGCCGCTACCTCTGAATGCAGTCGCCGAGAAGGGCATCG
TTGAAGTCGAGCAGGTTGGTGAAGCAATCCGTCGTGCGGTAAGTCGTTCTGGAACTAAGGCCAAATTTGCCGCTGCTGCG
GTTGCCGGTTCGGCAGTGATCACCAAGTTGATACCGATGCCTGCTGGTCTGGAGGAGCAGGATTTGGAAGCCCAGATAGA
AATAGAGGCTACGAATTACATCCCGTACCCTATTGAAGAGGTCAGCCTAGATTTCGAGGTGCTTGGTCCGGTTCCCAACA
ACACGGAGATGGTCCAAGTTTTGCTGGCTGCGTCTCGTTCGGAGAATGTCGAATTGCGCCAATCTGCGTTGGAGTTGGGT
GGGTTGACTGCCAAGGTTATTGACGTGGAAGCTTTAGCGGTTGAGAACGCTTTCTCTCTTATTGCCCAAGAACTGTCGGT
TGGGAGTAATGCATTGGTTGCATTGATCGACATCGGTGCAACGATGTCGACGCTTAATGTGCTGCATAGTGGTCGCAGTT
TGTACGCGCGCGAGCAGCTATTTGGTGGTAAGCAGCTTACTGACGAAGTGATGCATCGCTATGGGATGACTTACGAGGAG
GCTGGTCAGGCCAAGCGTCAAGGAGGTTTGCCGCAAAGCTATGATGTTGAAGTGCTGGGACCATTTAAGGACTCTGTGAT
ACAGCAGATCAGCCGTTTATTACAGTTTTTCTATGCTGGCAGTGAGTACAACCGAGTTGATTGCATCGTGTTGGCGGGAG
GCTGTGCTGTGATTGCGGGGTTACCGGCTATGGTCGAAGAACGGTTGGGTGTCGTTACCGTTGTGGCTAATCCGCTTGCG
CAGATGACTTTAGGGGCGAAAGTGCAAGCACACACGCTTGCTCAAGATGCACCTGCTTTAATGATTGCTACTGGTCTGGC
TTTGAGGAGTTTTGACTGA

Domains

Predicted by InterProScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

48.58

100

0.486
  comM Acinetobacter nosocomialis M2

48.58

100

0.486
  comM/pilM Acinetobacter baylyi ADP1

48.011

100

0.48
  pilM Legionella pneumophila strain ERS1305867

45.326

100

0.455