Detailed information NTG0815636

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: RYQ61_RS02610
• Genome accession: NZ_CP136799
• Coordinates: 517099..517536 (-)
• Length: 145 a.a.
• NCBI ID: WP_003073990.1
• Uniprot ID: T1ZH04
• Organism: Streptococcus intermedius strain XH2169
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 512099..522536

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RYQ61_RS02590 (RYQ61_02590)	glmS	512298..514112 (-)	1815	WP_021003253.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-
RYQ61_RS02595 (RYQ61_02595)	-	514502..515701 (-)	1200	WP_021003254.1	acetate kinase	-
RYQ61_RS02600 (RYQ61_02600)	comGH	515748..516701 (-)	954	WP_102568323.1	class I SAM-dependent methyltransferase	Machinery gene
RYQ61_RS02605 (RYQ61_02605)	comGG/cglG	516792..517118 (-)	327	WP_020999196.1	competence type IV pilus minor pilin ComGG	Machinery gene
RYQ61_RS02610 (RYQ61_02610)	comGF/cglF	517099..517536 (-)	438	WP_003073990.1	competence type IV pilus minor pilin ComGF	Machinery gene
RYQ61_RS02615 (RYQ61_02615)	comGE/cglE	517520..517813 (-)	294	WP_048785663.1	competence type IV pilus minor pilin ComGE	Machinery gene
RYQ61_RS02620 (RYQ61_02620)	comGD	517785..518213 (-)	429	WP_037582814.1	competence type IV pilus minor pilin ComGD	Machinery gene
RYQ61_RS02625 (RYQ61_02625)	comGC	518173..518490 (-)	318	WP_003073983.1	competence type IV pilus major pilin ComGC	Machinery gene
RYQ61_RS02630 (RYQ61_02630)	comGB	518487..519509 (-)	1023	WP_172964131.1	competence type IV pilus assembly protein ComGB	Machinery gene
RYQ61_RS02635 (RYQ61_02635)	comGA	519451..520392 (-)	942	WP_102568325.1	competence type IV pilus ATPase ComGA	Machinery gene
RYQ61_RS02640 (RYQ61_02640)	-	520461..520826 (-)	366	WP_048785667.1	DUF1033 family protein	-
RYQ61_RS02645 (RYQ61_02645)	glnA	521048..522394 (-)	1347	WP_021003260.1	type I glutamate--ammonia ligase	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16858.51 Da
• Isoelectric Point: 10.2149

>NTDB_id=815636 RYQ61_RS02610 WP_003073990.1 517099..517536(-) (comGF/cglF) [Streptococcus intermedius strain XH2169]
MFKANKVKAFTLLEALIALFVLSGSVLLFQSMTRLLASEVQTRQHSEQREWLLFADQLEVELTRSSFEKVENNRLYMKQD
GKVIAFGKASGQDFRKTNANGKGYQPMVYDVKKAEISQENELVRIRLLFKRGLEREFIYRVETKS

Nucleotide

• Length: 438 bp

>NTDB_id=815636 RYQ61_RS02610 WP_003073990.1 517099..517536(-) (comGF/cglF) [Streptococcus intermedius strain XH2169]
GTGTTCAAAGCAAATAAAGTAAAAGCTTTTACACTTTTAGAGGCTCTGATTGCCCTATTCGTACTCAGTGGAAGTGTGTT
GCTATTTCAATCTATGACGCGTCTTCTGGCTTCTGAAGTCCAAACTCGACAACACAGTGAACAGCGAGAATGGCTTTTGT
TTGCCGATCAACTAGAAGTGGAGTTGACGCGTTCTTCATTTGAAAAAGTGGAGAATAATCGTTTATACATGAAGCAAGAT
GGTAAGGTAATTGCATTTGGAAAAGCAAGTGGACAGGATTTCAGGAAAACCAATGCGAATGGGAAAGGTTATCAGCCCAT
GGTTTATGATGTGAAAAAAGCAGAAATTTCACAGGAAAATGAGCTGGTTCGTATTCGATTACTGTTTAAGAGAGGATTGG
AAAGGGAATTCATCTATCGTGTGGAAACAAAAAGTTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (38-134)

3D structure

• AlphaFold DB: T1ZH04

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae R6	68.571	96.552	0.662
comGF/cglF	Streptococcus pneumoniae TIGR4	68.571	96.552	0.662
comGF/cglF	Streptococcus pneumoniae Rx1	68.571	96.552	0.662
comGF/cglF	Streptococcus pneumoniae D39	68.571	96.552	0.662
comGF/cglF	Streptococcus mitis NCTC 12261	67.857	96.552	0.655
comGF/cglF	Streptococcus mitis SK321	67.857	96.552	0.655
comGF	Streptococcus mutans UA159	41.135	97.241	0.4
comGF	Streptococcus mutans UA140	41.135	97.241	0.4
comGF	Lactococcus lactis subsp. cremoris KW2	42.963	93.103	0.4