Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   M2042_RS14930 Genome accession   NZ_AP023187
Coordinates   3103035..3104780 (-) Length   581 a.a.
NCBI ID   WP_248525351.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain HLBS-07     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3098035..3109780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2042_RS14910 (HLBS07_28470) - 3098724..3099563 (-) 840 WP_104979776.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  M2042_RS14915 (HLBS07_28480) - 3099633..3101144 (-) 1512 WP_025768079.1 AAA family ATPase -
  M2042_RS14920 (HLBS07_28490) aroB 3101197..3102297 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  M2042_RS14925 (HLBS07_28500) aroK 3102329..3102847 (-) 519 WP_005381319.1 shikimate kinase AroK -
  M2042_RS14930 (HLBS07_28510) pilQ 3103035..3104780 (-) 1746 WP_248525351.1 type IV pilus secretin PilQ family protein Machinery gene
  M2042_RS14935 (HLBS07_28520) pilP 3104806..3105321 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  M2042_RS14940 (HLBS07_28530) pilO 3105311..3105904 (-) 594 WP_064354357.1 type 4a pilus biogenesis protein PilO Machinery gene
  M2042_RS14945 (HLBS07_28540) pilN 3105897..3106472 (-) 576 WP_005381323.1 PilN domain-containing protein Machinery gene
  M2042_RS14950 (HLBS07_28550) pilM 3106456..3107472 (-) 1017 WP_005381324.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63728.79 Da        Isoelectric Point: 4.4809

>NTDB_id=81550 M2042_RS14930 WP_248525351.1 3103035..3104780(-) (pilQ) [Vibrio alginolyticus strain HLBS-07]
MKMRQGLTRTPRILTGLFSLWLFVFLAPLAAAEEVSSNALKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDEQLYLLDVKDFSTVVESVEVFRYTSTTRLVAHINEEYMHDYRLTGRFLEIKVAKIKPNEKAPDKSILEREGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGSISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGIRWGFTSINGSNTVGGSIENNLATIGLYNGEGGDDGGDGDGVGIDDFLNVNLAATSPNAT
SIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVTFKKAVLSLKVTPQI
TPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFRR
SYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=81550 M2042_RS14930 WP_248525351.1 3103035..3104780(-) (pilQ) [Vibrio alginolyticus strain HLBS-07]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATACTTACTGGGCTCTTCTCCTTATGGTTGTTTGTATTTTTAGC
CCCTTTAGCGGCAGCTGAAGAAGTTTCGTCCAATGCCCTAAAAAGTATCGACTTTCGAACTAATAAAGATAAAGACGCCG
TGATTGTCGTCGAATTGGCGTCACCGGCCGCGATTGTAGATATCAAGCGTGTACAGGAAGGGTTAAGTATCGATTTGCTT
AACACTTCAGTAAAAGACGAGCAATTGTATCTCCTAGACGTGAAAGACTTTTCGACTGTGGTTGAGAGCGTAGAAGTGTT
TCGCTATACCTCAACTACGCGCTTGGTTGCGCACATCAATGAAGAATACATGCATGATTACCGTTTAACGGGGCGTTTTT
TGGAAATCAAAGTAGCGAAGATCAAACCCAATGAGAAAGCCCCAGATAAAAGCATTTTAGAAAGAGAAGGTAAGCTTATT
TCGATTAACTTCCAAGATATTCCTGTGCGAAACGTCTTGCAGCTTATTGCTGATTACAACCAATTCAATTTGGTTGTCTC
CGACTCTGTAGAAGGCAACCTCACGCTTCGTCTTGATGGTGTCCCTTGGCAGCAAGTGCTGGATATTATTTTGCAAGTTA
AGGGGTTAGATAAGCGTGTTGATGGCAATGTGATTTTAGTTGCTCCGACTGATGAGTTGGACTTGCGTGAGAAGCAGCAG
TTAGAAAAACAAAAATTGACGGAAGAAATGGGAGAGCTTTCATCTGAAATCATCAAAGTTAATTTTGCTAAAGCATCAGA
TATTGCGGAGATGATCAATGGAGAAGGAAGTATCAGCATGCTGTCAGAGCGCGGCAGTATGACCATTGATGAGCGAACTA
ACTCGTTATTGATTAGAGAGTTACCTGAAAACATCGAGGTGATTCGAGAAATTATTGAGTCTTTGGATATTCCAGTTAAA
CAGGTTCAAATTGAGGCGCGTATTGTGACCATCAATGAAGGTAACATGGACGAGTTAGGGATTCGTTGGGGTTTTACATC
TATTAATGGCAGTAATACAGTGGGCGGCTCTATTGAGAACAACCTCGCCACGATCGGTCTTTATAATGGAGAAGGGGGCG
ACGACGGTGGTGACGGTGATGGAGTGGGGATCGATGACTTCCTAAATGTAAACTTAGCTGCAACCAGCCCGAATGCGACC
AGCATTGCTTTTCAGGTCGCAAAGTTAGGTTCAGACACCTTGTTGGATCTTGAGCTTTCTGCTCTGCAACAAGAGTCTAA
AGCTGAGATTATCTCGAGCCCACGTTTGATCACTACCAATAAGAAACCAGCCTATATCGAACAAGGTACAGAGATTCCTT
ACCTAGAGTCATCATCAAGTGGTGCAACGACGGTGACGTTCAAAAAGGCGGTATTAAGTTTGAAGGTGACCCCGCAAATC
ACACCGGATAACCGCTTAGTGTTGGATTTAAGTGTCACGCAAGATAGACCTGGACAAGTCGTGAAGACAGGCACTGGTGA
AGCTGTTGCGATTGATACTCAACGTATCGGGACGCAAGTGTTAGTGAATAACGGTGAAACGGTCGTTCTTGGTGGTATTT
TTCAGCACAGCATTACTAACTCGGTGGACAAAGTGCCTTTGTTGGGCGATCTGCCGCTATTAGGTGCGCTTTTTCGTCGA
AGCTATGAAAATGTTGGCAAAAGTGAGCTGTTAATTTTCGTCACGCCTAAGGTGGTGATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(421-579)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

92.599

100

0.926
  pilQ Vibrio cholerae strain A1552

74.57

100

0.747
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.57

100

0.747
  pilQ Pseudomonas aeruginosa PAK

39.896

99.656

0.398
  pilQ Legionella pneumophila strain Lp02

40.429

96.213

0.389
  pilQ Legionella pneumophila strain ERS1305867

40.429

96.213

0.389
  comQ Acinetobacter baylyi ADP1

37.241

99.828

0.372
  pilQ Acinetobacter baumannii D1279779

35.738

100

0.367
  pilQ Acinetobacter baumannii strain A118

35.738

100

0.367

Multiple sequence alignment