Detailed information NTG0815044

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: RZV12_RS14940
• Genome accession: NZ_CP136552
• Coordinates: 3023195..3024205 (-)
• Length: 336 a.a.
• NCBI ID: WP_000568519.1
• Uniprot ID: Q32HA1
• Organism: Escherichia coli O104:H4 strain rpoS_ATGtoATA
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 3018195..3029205

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RZV12_RS14920	mepM	3019162..3020484 (-)	1323	WP_001184045.1	murein DD-endopeptidase MepM	-
RZV12_RS14925	znuA	3020500..3021432 (-)	933	WP_001342995.1	zinc ABC transporter substrate-binding protein ZnuA	-
RZV12_RS14930	znuC	3021511..3022266 (+)	756	WP_000202996.1	zinc ABC transporter ATP-binding protein ZnuC	-
RZV12_RS14935	znuB	3022263..3023048 (+)	786	WP_000571465.1	zinc ABC transporter permease subunit ZnuB	-
RZV12_RS14940	ruvB	3023195..3024205 (-)	1011	WP_000568519.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
RZV12_RS14945	ruvA	3024214..3024825 (-)	612	WP_000580323.1	Holliday junction branch migration protein RuvA	-
RZV12_RS14950	yobI	3024964..3025029 (-)	66	WP_001386853.1	stress response small protein YobI	-
RZV12_RS14955	yebB	3025100..3025702 (+)	603	WP_001024930.1	YebB family permuted papain-like enzyme	-
RZV12_RS14960	ruvC	3025704..3026225 (-)	522	WP_001295503.1	crossover junction endodeoxyribonuclease RuvC	-
RZV12_RS14965	yebC	3026260..3027000 (-)	741	WP_000907234.1	YebC/PmpR family DNA-binding transcriptional regulator	-
RZV12_RS14970	nudB	3027029..3027481 (-)	453	WP_077221229.1	dihydroneopterin triphosphate diphosphatase	-

Sequence

Protein

• Length: 336 a.a.
• Molecular weight: 37173.77 Da
• Isoelectric Point: 4.7818

>NTDB_id=815044 RZV12_RS14940 WP_000568519.1 3023195..3024205(-) (ruvB) [Escherichia coli O104:H4 strain rpoS_ATGtoATA]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide

• Length: 1011 bp

>NTDB_id=815044 RZV12_RS14940 WP_000568519.1 3023195..3024205(-) (ruvB) [Escherichia coli O104:H4 strain rpoS_ATGtoATA]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTCGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATCGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGAGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

3D structure

• AlphaFold DB: Q32HA1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.486	97.917	0.592
ruvB	Streptococcus pneumoniae TIGR4	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae R6	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae D39	58.934	94.94	0.56
ruvB	Synechocystis sp. PCC 6803	53.251	96.131	0.512
ruvB	Helicobacter pylori 26695	52.038	94.94	0.494