Detailed information NTG0813567

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MTS25_RS14605
• Genome accession: NZ_CP121629
• Coordinates: 3025678..3026796 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain JAB270
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3020678..3031796

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MTS25_RS14595 (MTS25_14595)	-	3023794..3024486 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
MTS25_RS14600 (MTS25_14600)	pilT	3024613..3025650 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
MTS25_RS14605 (MTS25_14605)	pilU	3025678..3026796 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MTS25_RS14610 (MTS25_14610)	fur	3026866..3027303 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
MTS25_RS14615 (MTS25_14615)	bamE	3027415..3027813 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
MTS25_RS14620 (MTS25_14620)	-	3027868..3028191 (-)	324	WP_000442587.1	RnfH family protein	-
MTS25_RS14625 (MTS25_14625)	-	3028191..3029258 (-)	1068	WP_002019572.1	hypothetical protein	-
MTS25_RS14630 (MTS25_14630)	-	3029411..3029866 (-)	456	WP_000782976.1	bacteriohemerythrin	-
MTS25_RS14635 (MTS25_14635)	-	3030119..3030967 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=813567 MTS25_RS14605 WP_000347039.1 3025678..3026796(+) (pilU) [Acinetobacter baumannii strain JAB270]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=813567 MTS25_RS14605 WP_000347039.1 3025678..3026796(+) (pilU) [Acinetobacter baumannii strain JAB270]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395