Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MTS88_RS12300 Genome accession   NZ_CP121560
Coordinates   2494330..2495448 (+) Length   372 a.a.
NCBI ID   WP_000347039.1    Uniprot ID   A0A9P2XLL8
Organism   Acinetobacter baumannii strain RAB97     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2485348..2531990 2494330..2495448 within 0


Gene organization within MGE regions


Location: 2485348..2531990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTS88_RS12265 (MTS88_12265) - 2485456..2485908 (-) 453 WP_000164153.1 ABZJ_00895 family protein -
  MTS88_RS12270 (MTS88_12270) - 2486288..2486665 (+) 378 WP_001059438.1 VOC family protein -
  MTS88_RS12275 (MTS88_12275) - 2486733..2487206 (-) 474 WP_000974358.1 OsmC family protein -
  MTS88_RS12280 (MTS88_12280) - 2487370..2488638 (+) 1269 WP_000116701.1 exonuclease SbcCD subunit D -
  MTS88_RS12285 (MTS88_12285) - 2488648..2492244 (+) 3597 WP_000698785.1 SbcC/MukB-like Walker B domain-containing protein -
  MTS88_RS12290 (MTS88_12290) - 2492446..2493138 (-) 693 WP_001108512.1 YggS family pyridoxal phosphate-dependent enzyme -
  MTS88_RS12295 (MTS88_12295) pilT 2493265..2494302 (+) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  MTS88_RS12300 (MTS88_12300) pilU 2494330..2495448 (+) 1119 WP_000347039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MTS88_RS12305 (MTS88_12305) fur 2495518..2495955 (-) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  MTS88_RS12310 (MTS88_12310) bamE 2496067..2496465 (+) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  MTS88_RS12315 (MTS88_12315) - 2496520..2496843 (-) 324 WP_000442587.1 RnfH family protein -
  MTS88_RS12320 (MTS88_12320) - 2496843..2497910 (-) 1068 WP_002019572.1 hypothetical protein -
  MTS88_RS12325 (MTS88_12325) - 2498063..2498518 (-) 456 WP_000782976.1 bacteriohemerythrin -
  MTS88_RS12330 (MTS88_12330) - 2498771..2499619 (-) 849 WP_000336548.1 class II glutamine amidotransferase -
  MTS88_RS12335 (MTS88_12335) - 2499728..2500510 (-) 783 WP_000890283.1 GNAT family N-acetyltransferase -
  MTS88_RS12340 (MTS88_12340) argB 2500631..2501539 (-) 909 WP_001135419.1 acetylglutamate kinase -
  MTS88_RS12345 (MTS88_12345) - 2501554..2502972 (-) 1419 WP_001102845.1 phosphomannomutase/phosphoglucomutase -
  MTS88_RS12350 (MTS88_12350) dut 2503172..2503624 (-) 453 WP_000868152.1 dUTP diphosphatase -
  MTS88_RS12355 (MTS88_12355) rep 2503649..2505688 (-) 2040 WP_000093035.1 DNA helicase Rep -
  MTS88_RS12360 (MTS88_12360) - 2505882..2506535 (-) 654 WP_001202415.1 OmpA family protein -
  MTS88_RS12365 (MTS88_12365) - 2506780..2507706 (+) 927 WP_000100965.1 acyltransferase -
  MTS88_RS12370 (MTS88_12370) - 2507723..2508769 (+) 1047 WP_001181639.1 hypothetical protein -
  MTS88_RS12375 (MTS88_12375) minC 2508891..2509613 (+) 723 WP_000763677.1 septum site-determining protein MinC -
  MTS88_RS12380 (MTS88_12380) minD 2509684..2510496 (+) 813 WP_001074362.1 septum site-determining protein MinD -
  MTS88_RS12385 (MTS88_12385) minE 2510499..2510771 (+) 273 WP_000896934.1 cell division topological specificity factor MinE -
  MTS88_RS12390 (MTS88_12390) - 2510855..2511142 (+) 288 WP_001218560.1 PA4642 family protein -
  MTS88_RS12395 (MTS88_12395) - 2511290..2511457 (+) 168 Protein_2435 tyrosyl-tRNA synthetase -
  MTS88_RS12400 (MTS88_12400) - 2511698..2512666 (+) 969 WP_012308653.1 IS30 family transposase -
  MTS88_RS12405 (MTS88_12405) - 2512674..2512928 (+) 255 Protein_2437 hypothetical protein -
  MTS88_RS12410 (MTS88_12410) - 2512971..2513898 (+) 928 Protein_2438 IS5-like element ISAba10 family transposase -
  MTS88_RS12415 (MTS88_12415) - 2513953..2514174 (+) 222 Protein_2439 tyrosyl-tRNA synthetase -
  MTS88_RS12420 (MTS88_12420) rhtC 2514230..2514850 (-) 621 WP_000959259.1 threonine export protein RhtC -
  MTS88_RS12425 (MTS88_12425) - 2515000..2518032 (-) 3033 WP_000127823.1 DNA translocase FtsK -
  MTS88_RS12430 (MTS88_12430) trxB 2518302..2519249 (+) 948 WP_001276144.1 thioredoxin-disulfide reductase -
  MTS88_RS12435 (MTS88_12435) aat 2519369..2520100 (+) 732 WP_000854785.1 leucyl/phenylalanyl-tRNA--protein transferase -
  MTS88_RS12440 (MTS88_12440) - 2520119..2520934 (+) 816 WP_000844343.1 arginyltransferase -
  MTS88_RS12445 (MTS88_12445) rimI 2520979..2521434 (-) 456 WP_000621543.1 ribosomal protein S18-alanine N-acetyltransferase -
  MTS88_RS12450 (MTS88_12450) - 2521543..2522418 (-) 876 WP_000992291.1 metal-dependent hydrolase -
  MTS88_RS12455 (MTS88_12455) - 2522655..2523536 (-) 882 WP_000992302.1 metal-dependent hydrolase -
  MTS88_RS12460 (MTS88_12460) tuf 2523740..2524930 (-) 1191 WP_001029610.1 elongation factor Tu -
  MTS88_RS12465 (MTS88_12465) fusA 2525025..2527163 (-) 2139 WP_243327632.1 elongation factor G -
  MTS88_RS12470 (MTS88_12470) rpsG 2527342..2527812 (-) 471 WP_001138055.1 30S ribosomal protein S7 -
  MTS88_RS12475 (MTS88_12475) rpsL 2527972..2528346 (-) 375 WP_000246374.1 30S ribosomal protein S12 -
  MTS88_RS12480 (MTS88_12480) - 2528547..2529872 (-) 1326 WP_000160010.1 EcsC family protein -
  MTS88_RS12485 (MTS88_12485) - 2530169..2531398 (+) 1230 WP_000832710.1 beta-ketoacyl-ACP synthase II -
  MTS88_RS12490 (MTS88_12490) hemJ 2531417..2531869 (+) 453 WP_000339890.1 protoporphyrinogen oxidase HemJ -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41933.13 Da        Isoelectric Point: 6.6944

>NTDB_id=812948 MTS88_RS12300 WP_000347039.1 2494330..2495448(+) (pilU) [Acinetobacter baumannii strain RAB97]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=812948 MTS88_RS12300 WP_000347039.1 2494330..2495448(+) (pilU) [Acinetobacter baumannii strain RAB97]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

69.565

98.925

0.688
  pilU Vibrio cholerae strain A1552

53.243

99.462

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395