Detailed information NTG0812671

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: RUW23_RS15585
• Genome accession: NZ_CP136014
• Coordinates: 3317192..3318256 (+)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: A0A0H2ZID6
• Organism: Pseudomonas aeruginosa strain R18-20
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3312192..3323256

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RUW23_RS15575 (RUW23_15575)	-	3313163..3314431 (+)	1269	WP_003103278.1	malic enzyme	-
RUW23_RS15580 (RUW23_15580)	-	3314536..3317007 (-)	2472	WP_031756300.1	penicillin-binding protein 1A	-
RUW23_RS15585 (RUW23_15585)	pilM	3317192..3318256 (+)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
RUW23_RS15590 (RUW23_15590)	pilN	3318256..3318852 (+)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	Machinery gene
RUW23_RS15595 (RUW23_15595)	pilO	3318849..3319472 (+)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	Machinery gene
RUW23_RS15600 (RUW23_15600)	comL/pilP	3319469..3319993 (+)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	Machinery gene
RUW23_RS15605 (RUW23_15605)	pilQ	3320047..3322191 (+)	2145	WP_003123619.1	type 4a pilus secretin PilQ	Machinery gene
RUW23_RS15610 (RUW23_15610)	aroK	3322203..3322721 (+)	519	WP_003095833.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=812671 RUW23_RS15585 WP_003110888.1 3317192..3318256(+) (pilM) [Pseudomonas aeruginosa strain R18-20]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=812671 RUW23_RS15585 WP_003110888.1 3317192..3318256(+) (pilM) [Pseudomonas aeruginosa strain R18-20]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAAAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0H2ZID6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM/pilM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466