Detailed information NTG0081207

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSm6_RS12090
• Genome accession: NZ_AP023081
• Coordinates: 2669866..2671011 (+)
• Length: 381 a.a.
• NCBI ID: WP_021218362.1
• Uniprot ID: A0AAU7Y6D2
• Organism: Pseudomonas solani strain Sm006
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2664866..2676011

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSm6_RS12065 (PSm6_24250)	-	2665131..2665724 (-)	594	WP_265170282.1	YggT family protein	-
PSm6_RS12070 (PSm6_24260)	-	2665887..2667101 (-)	1215	WP_265170283.1	hypothetical protein	-
PSm6_RS12075 (PSm6_24270)	proC	2667107..2667925 (-)	819	WP_021218359.1	pyrroline-5-carboxylate reductase	-
PSm6_RS12080 (PSm6_24290)	-	2667939..2668633 (-)	695	Protein_2380	YggS family pyridoxal phosphate-dependent enzyme	-
PSm6_RS12085 (PSm6_24300)	pilT	2668702..2669736 (+)	1035	WP_021218361.1	type IV pilus twitching motility protein PilT	Machinery gene
PSm6_RS12090 (PSm6_24320)	pilU	2669866..2671011 (+)	1146	WP_021218362.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSm6_RS12095 (PSm6_24330)	-	2670944..2672437 (-)	1494	WP_265170285.1	UdgX family uracil-DNA binding protein	-
PSm6_RS12100 (PSm6_24340)	-	2672437..2673651 (-)	1215	WP_265170287.1	putative DNA modification/repair radical SAM protein	-
PSm6_RS12105 (PSm6_24350)	-	2673753..2674148 (+)	396	WP_021218365.1	TM2 domain-containing protein	-
PSm6_RS12110 (PSm6_24360)	-	2674208..2675479 (-)	1272	WP_265170288.1	dihydroorotase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42463.75 Da
• Isoelectric Point: 6.5931

>NTDB_id=81207 PSm6_RS12090 WP_021218362.1 2669866..2671011(+) (pilU) [Pseudomonas solani strain Sm006]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNAMSPEQTRETVLAVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLDDLKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYTLYSQGEITYEDALLYADSANDLRLMIKLGSETDGDHLTSMSGGLSLEVSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=81207 PSm6_RS12090 WP_021218362.1 2669866..2671011(+) (pilU) [Pseudomonas solani strain Sm006]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTCGAGAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTCCCCCCGTC
GATGAAGGTCAACGGCAAGATCATGCCCGTGACCAAGAACGCCATGTCCCCGGAGCAGACCCGTGAAACCGTTCTCGCGG
TGATGAACGAGCAGCAGCGCCGTGACTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGGGGCATCGGCCGT
TTCCGCGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCCACCCTGGA
TGACCTCAAGCTGCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGCTTTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACTTCCCTGGCGGCGATGATCGGCTACCGCAACAAGAACAGCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAGTACATCCACCAGCACCAGAGCTGCATCGTCACCCAGCGTGAAGTGGGCATCGACACCGAGTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGTGAGGTGCGGACCCGCGAAACCATGGACCATG
CCGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCTGCCGACCGTCAGCAGCAGGTGTGGATGGACCTGTCCCTCAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGACGGCAAGGGCCGCCGCGCGGTGATCGAGGTGCTGATCAACACCCCGCTGGCTGCCGACCTGA
TCCGCAAGGGCGAGGTCCATGAGCTCAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACACCCTCTACTCCCAGGGCGAGATCACTTACGAAGACGCGCTGCTCTACGCCGACTCGGCGAACGACCTGCGCCT
GATGATCAAGCTCGGTTCCGAAACCGACGGCGACCACCTCACGAGCATGAGCGGTGGCCTGTCGCTGGAAGTGTCCGAGG
AAGACCCGGGCCGTCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	91.339	100	0.913
pilU	Acinetobacter baylyi ADP1	64.426	93.701	0.604
pilU	Vibrio cholerae strain A1552	58.857	91.864	0.541
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.155	88.189	0.381