Detailed information NTG0081109

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: OPA53_RS10825
• Genome accession: NZ_AP026794
• Coordinates: 2231720..2232730 (+)
• Length: 336 a.a.
• NCBI ID: WP_000568520.1
• Uniprot ID: -
• Organism: Escherichia coli strain IPCEC48
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2226720..2237730

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OPA53_RS10795 (IPCEC48_20830)	nudB	2228444..2228896 (+)	453	WP_077249130.1	dihydroneopterin triphosphate diphosphatase	-
OPA53_RS10800 (IPCEC48_20840)	yebC	2228925..2229665 (+)	741	WP_000907234.1	YebC/PmpR family DNA-binding transcriptional regulator	-
OPA53_RS10805 (IPCEC48_20850)	ruvC	2229700..2230221 (+)	522	WP_001295503.1	crossover junction endodeoxyribonuclease RuvC	-
OPA53_RS10810 (IPCEC48_20860)	yebB	2230223..2230825 (-)	603	WP_001024911.1	YebB family permuted papain-like enzyme	-
OPA53_RS10815	yobI	2230896..2230961 (+)	66	WP_072093883.1	stress response small protein YobI	-
OPA53_RS10820 (IPCEC48_20870)	ruvA	2231100..2231711 (+)	612	WP_000580323.1	Holliday junction branch migration protein RuvA	-
OPA53_RS10825 (IPCEC48_20880)	ruvB	2231720..2232730 (+)	1011	WP_000568520.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
OPA53_RS10830	-	2232840..2234188 (+)	1349	WP_099156422.1	IS3-like element IS1397 family transposase	-
OPA53_RS10835 (IPCEC48_20910)	znuB	2234382..2235167 (-)	786	WP_000571478.1	zinc ABC transporter permease subunit ZnuB	-
OPA53_RS10840 (IPCEC48_20920)	znuC	2235164..2235919 (-)	756	WP_000202996.1	zinc ABC transporter ATP-binding protein ZnuC	-
OPA53_RS10845 (IPCEC48_20930)	znuA	2235998..2236930 (+)	933	WP_001347089.1	zinc ABC transporter substrate-binding protein ZnuA	-

Sequence

Protein

• Length: 336 a.a.
• Molecular weight: 37163.73 Da
• Isoelectric Point: 4.7818

>NTDB_id=81109 OPA53_RS10825 WP_000568520.1 2231720..2232730(+) (ruvB) [Escherichia coli strain IPCEC48]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMS

Nucleotide

• Length: 1011 bp

>NTDB_id=81109 OPA53_RS10825 WP_000568520.1 2231720..2232730(+) (ruvB) [Escherichia coli strain IPCEC48]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTATTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAATATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCAATGCTCACTAACCTTGAACCACA
CGATGTACTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGTGCGCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGCGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGTCGTAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.486	97.917	0.592
ruvB	Streptococcus pneumoniae TIGR4	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae R6	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae D39	58.934	94.94	0.56
ruvB	Synechocystis sp. PCC 6803	53.251	96.131	0.512
ruvB	Helicobacter pylori 26695	52.038	94.94	0.494