Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   RS172_RS10145 Genome accession   NZ_CP135646
Coordinates   2123590..2124600 (+) Length   336 a.a.
NCBI ID   WP_000568520.1    Uniprot ID   -
Organism   Escherichia coli strain TUM14668     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2118590..2129600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS172_RS10115 (RS172_10115) nudB 2120314..2120766 (+) 453 WP_077249130.1 dihydroneopterin triphosphate diphosphatase -
  RS172_RS10120 (RS172_10120) yebC 2120795..2121535 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  RS172_RS10125 (RS172_10125) ruvC 2121570..2122091 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  RS172_RS10130 (RS172_10130) yebB 2122093..2122695 (-) 603 WP_001024911.1 YebB family permuted papain-like enzyme -
  RS172_RS10135 (RS172_10135) yobI 2122766..2122831 (+) 66 WP_072093883.1 stress response small protein YobI -
  RS172_RS10140 (RS172_10140) ruvA 2122970..2123581 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  RS172_RS10145 (RS172_10145) ruvB 2123590..2124600 (+) 1011 WP_000568520.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  RS172_RS10150 (RS172_10150) - 2124710..2126058 (+) 1349 WP_099156422.1 IS3-like element IS1397 family transposase -
  RS172_RS10155 (RS172_10155) znuB 2126252..2127037 (-) 786 WP_000571478.1 zinc ABC transporter permease subunit ZnuB -
  RS172_RS10160 (RS172_10160) znuC 2127034..2127789 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  RS172_RS10165 (RS172_10165) znuA 2127868..2128800 (+) 933 WP_001347089.1 zinc ABC transporter substrate-binding protein ZnuA -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37163.73 Da        Isoelectric Point: 4.7818

>NTDB_id=810964 RS172_RS10145 WP_000568520.1 2123590..2124600(+) (ruvB) [Escherichia coli strain TUM14668]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMS

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=810964 RS172_RS10145 WP_000568520.1 2123590..2124600(+) (ruvB) [Escherichia coli strain TUM14668]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTATTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAATATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCAATGCTCACTAACCTTGAACCACA
CGATGTACTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGTGCGCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGCGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGTCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494