Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   QN329_RS01015 Genome accession   NZ_CP135103
Coordinates   178660..179115 (-) Length   151 a.a.
NCBI ID   WP_000793378.1    Uniprot ID   Q8E234
Organism   Streptococcus agalactiae strain HU36/21     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 173660..184115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QN329_RS00980 (QN329_00980) - 174214..174879 (-) 666 WP_000008107.1 CPBP family intramembrane glutamic endopeptidase -
  QN329_RS00985 (QN329_00985) - 174948..175340 (-) 393 WP_001265365.1 hypothetical protein -
  QN329_RS00990 (QN329_00990) - 175443..175580 (-) 138 WP_079263416.1 hypothetical protein -
  QN329_RS00995 (QN329_00995) - 175639..175845 (-) 207 WP_000798241.1 helix-turn-helix transcriptional regulator -
  QN329_RS01000 (QN329_01000) - 175997..177190 (-) 1194 WP_000047537.1 acetate kinase -
  QN329_RS01005 (QN329_01005) comGH 177222..178196 (-) 975 WP_001008568.1 class I SAM-dependent methyltransferase Machinery gene
  QN329_RS01010 (QN329_01010) comGG 178311..178682 (-) 372 WP_000601103.1 competence type IV pilus minor pilin ComGG Machinery gene
  QN329_RS01015 (QN329_01015) comGF 178660..179115 (-) 456 WP_000793378.1 competence type IV pilus minor pilin ComGF Machinery gene
  QN329_RS01020 (QN329_01020) comGB 179112..180155 (-) 1044 WP_228322108.1 competence type IV pilus assembly protein ComGB Machinery gene
  QN329_RS01025 (QN329_01025) comGA 180049..181020 (-) 972 WP_000250799.1 competence type IV pilus ATPase ComGA Machinery gene
  QN329_RS01030 (QN329_01030) - 181192..181557 (-) 366 WP_000285373.1 DUF1033 family protein -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 17509.47 Da        Isoelectric Point: 9.7378

>NTDB_id=809902 QN329_RS01015 WP_000793378.1 178660..179115(-) (comGF) [Streptococcus agalactiae strain HU36/21]
MKNLLLKCKDKKVKAFTLLECLVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQNK
LYLRKQDKIVTFGKSNKDDFRKTGYDGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET

Nucleotide


Download         Length: 456 bp        

>NTDB_id=809902 QN329_RS01015 WP_000793378.1 178660..179115(-) (comGF) [Streptococcus agalactiae strain HU36/21]
ATGAAAAATTTATTGTTAAAATGTAAGGATAAGAAGGTTAAAGCATTTACGCTTTTAGAATGTTTGGTAGCATTGGTTAC
AATTACAGGAGCTTTACTAGTTTATCAAGGACTGACAAAATTGTTGGCTCAACAGATAGTAGTGATGTCTTCTTCCAGTC
AGTCTGAATGGGTGTTATTAACTCAGCAACTAAATGCAGAATTTGAAGGCGCTCATCTGGAATATTTAAGACAGAACAAA
CTTTATTTACGTAAGCAAGATAAGATTGTAACCTTTGGCAAATCTAATAAAGATGATTTCCGTAAGACAGGTTATGATGG
TCGAGGTTATCAACCAATGGTTTATGGGTTAGACAATTGTCAAATGAGTCAAACCAAAAGTATGGTAAAACTTGTTTTTT
ATTTTAAGGACGGGTTAAAAAGGACATTTTACTATGATTTTAAAGAAGAAACTTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(47-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8E234

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Streptococcus mutans UA159

48.571

92.715

0.45
  comGF Streptococcus mutans UA140

48.571

92.715

0.45
  comGF/cglF Streptococcus pneumoniae Rx1

42

99.338

0.417
  comGF/cglF Streptococcus pneumoniae D39

42

99.338

0.417
  comGF/cglF Streptococcus pneumoniae R6

42

99.338

0.417
  comGF/cglF Streptococcus pneumoniae TIGR4

42

99.338

0.417
  comGF/cglF Streptococcus mitis SK321

44.203

91.391

0.404
  comGF/cglF Streptococcus mitis NCTC 12261

43.478

91.391

0.397
  comGF Lactococcus lactis subsp. cremoris KW2

40.58

91.391

0.371