Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   P7249_RS02485 Genome accession   NZ_CP120944
Coordinates   501388..502512 (+) Length   374 a.a.
NCBI ID   WP_000228544.1    Uniprot ID   A0A9P2PX49
Organism   Escherichia coli O157:H7 str. EDL933     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 496388..507512
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7249_RS02460 mscL 496514..496924 (-) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -
  P7249_RS02465 trkA 497054..498430 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  P7249_RS02470 rsmB 498452..499741 (-) 1290 WP_001301795.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  P7249_RS02475 fmt 499787..500734 (-) 948 WP_000004459.1 methionyl-tRNA formyltransferase -
  P7249_RS02480 def 500749..501258 (-) 510 WP_000114984.1 peptide deformylase -
  P7249_RS02485 dprA 501388..502512 (+) 1125 WP_000228544.1 DNA-protecting protein DprA Machinery gene
  P7249_RS02490 smg 502484..502957 (+) 474 WP_000460672.1 DUF494 family protein Smg -
  P7249_RS02495 yrdD 502986..503528 (+) 543 WP_001129722.1 type I DNA topoisomerase -
  P7249_RS02500 tsaC 503533..504105 (+) 573 WP_001301437.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  P7249_RS02505 aroE 504110..504928 (+) 819 WP_000451254.1 shikimate dehydrogenase -
  P7249_RS02510 yrdB 504925..505182 (+) 258 WP_001070590.1 DUF1488 domain-containing protein -
  P7249_RS02515 yrdA 505158..505712 (-) 555 WP_001286216.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41005.96 Da        Isoelectric Point: 6.2655

>NTDB_id=809492 P7249_RS02485 WP_000228544.1 501388..502512(+) (dprA) [Escherichia coli O157:H7 str. EDL933]
MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=809492 P7249_RS02485 WP_000228544.1 501388..502512(+) (dprA) [Escherichia coli O157:H7 str. EDL933]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAGACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGGAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGTGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGAAGCATGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTAGCGCATAAAGCAGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCT
TGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGTAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGAGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Glaesserella parasuis strain SC1401

45.401

90.107

0.409
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Haemophilus influenzae Rd KW20

44.214

90.107

0.398
  dprA Legionella pneumophila strain ERS1305867

44.545

88.235

0.393