Detailed information NTG0809105

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P4G01_RS02280
• Genome accession: NZ_CP120881
• Coordinates: 469178..470326 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain NY7583
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 464178..475326

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P4G01_RS02255 (P4G01_02255)	-	465329..465625 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
P4G01_RS02260 (P4G01_02260)	-	465622..466215 (-)	594	WP_003084544.1	YggT family protein	-
P4G01_RS02265 (P4G01_02265)	proC	466226..467047 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
P4G01_RS02270 (P4G01_02270)	-	467059..467751 (-)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
P4G01_RS02275 (P4G01_02275)	pilT	467966..469000 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
P4G01_RS02280 (P4G01_02280)	pilU	469178..470326 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
P4G01_RS02285 (P4G01_02285)	czcD	470334..471233 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
P4G01_RS02290 (P4G01_02290)	-	471387..471791 (+)	405	WP_003084560.1	NINE protein	-
P4G01_RS02295 (P4G01_02295)	-	472034..473407 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
P4G01_RS02300 (P4G01_02300)	-	473404..474588 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=809105 P4G01_RS02280 WP_003100959.1 469178..470326(+) (pilU) [Pseudomonas aeruginosa strain NY7583]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=809105 P4G01_RS02280 WP_003100959.1 469178..470326(+) (pilU) [Pseudomonas aeruginosa strain NY7583]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382