Detailed information NTG0809020

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P4B34_RS32055
• Genome accession: NZ_CP120859
• Coordinates: 6690021..6691169 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain NY4383
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6685021..6696169

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P4B34_RS32035 (P4B34_32055)	-	6685758..6686942 (-)	1185	WP_003084564.1	cystathionine gamma-synthase	-
P4B34_RS32040 (P4B34_32060)	-	6686939..6688312 (-)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
P4B34_RS32045 (P4B34_32065)	-	6688555..6688959 (-)	405	WP_003084560.1	NINE protein	-
P4B34_RS32050 (P4B34_32070)	czcD	6689114..6690013 (+)	900	WP_003084557.1	CDF family zinc transporter CzcD	-
P4B34_RS32055 (P4B34_32075)	pilU	6690021..6691169 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
P4B34_RS32060 (P4B34_32080)	pilT	6691347..6692381 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
P4B34_RS32065 (P4B34_32085)	-	6692596..6693288 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
P4B34_RS32070 (P4B34_32090)	proC	6693300..6694121 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
P4B34_RS32075 (P4B34_32095)	-	6694132..6694725 (+)	594	WP_003084544.1	YggT family protein	-
P4B34_RS32080 (P4B34_32100)	-	6694722..6695018 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=809020 P4B34_RS32055 WP_003100959.1 6690021..6691169(-) (pilU) [Pseudomonas aeruginosa strain NY4383]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=809020 P4B34_RS32055 WP_003100959.1 6690021..6691169(-) (pilU) [Pseudomonas aeruginosa strain NY4383]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382