Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   P2A85_RS13975 Genome accession   NZ_CP120853
Coordinates   3256023..3257117 (-) Length   364 a.a.
NCBI ID   WP_341736464.1    Uniprot ID   -
Organism   MAG: Microcoleus anatoxicus isolate Neu2020_A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3251023..3262117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P2A85_RS13960 (P2A85_13900) - 3251622..3254006 (-) 2385 WP_377481058.1 AMIN domain-containing protein -
  P2A85_RS13965 (P2A85_13905) - 3254234..3255049 (-) 816 WP_377481061.1 pilus assembly protein PilO -
  P2A85_RS13970 (P2A85_13910) - 3255046..3255975 (-) 930 WP_377481064.1 PilN domain-containing protein -
  P2A85_RS13975 (P2A85_13915) pilM 3256023..3257117 (-) 1095 WP_341736464.1 type IV pilus assembly protein PilM Machinery gene
  P2A85_RS13980 (P2A85_13920) - 3257436..3258719 (+) 1284 WP_377481067.1 ABC transporter substrate-binding protein -
  P2A85_RS13985 (P2A85_13925) - 3259015..3259686 (+) 672 WP_377481070.1 ThiF family adenylyltransferase -
  P2A85_RS13990 (P2A85_13930) - 3259724..3260893 (+) 1170 WP_377481072.1 hypothetical protein -
  P2A85_RS13995 (P2A85_13935) - 3260901..3261146 (+) 246 WP_377481075.1 hypothetical protein -
  P2A85_RS14000 (P2A85_13940) - 3261222..3261968 (+) 747 WP_377481078.1 hypothetical protein -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 39827.49 Da        Isoelectric Point: 4.1191

>NTDB_id=808918 P2A85_RS13975 WP_341736464.1 3256023..3257117(-) (pilM) [MAG: Microcoleus anatoxicus isolate Neu2020_A]
MVNFFKNLISRRQPGIGIELASDRINIIQLEKKAQALKIATLASIEVPEDIVQEGQIADPPGMAELIRSVLAENKIKAKH
VATAIPGREAVIRLIPVPSELNNEELREYMNSEAGLYLPFPREDADVDYQKIGLFVDDDGVEKVQVVLVATRKEVTDTYL
ETFKEAGLIIDVLEVTSFALIRTLKDQLQQFSSQEAAVLTDIEFDSTELAIVVDGIPQFNRTIPIGTYQIQTALNEAMNL
PPTRDTSELQGMTLPVTDTMGTSGDANPGTNAMIKVLGELADELRRSVDFYLNQSEELEVAQLLLAGPGAAIGKLDEFFM
QRLSLPASQVDPIDTLGLEVSEEISPEQRAGLGVALGLGLREVL

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=808918 P2A85_RS13975 WP_341736464.1 3256023..3257117(-) (pilM) [MAG: Microcoleus anatoxicus isolate Neu2020_A]
ATGGTTAACTTTTTCAAAAATCTAATTTCCAGACGCCAGCCCGGAATCGGCATCGAACTAGCCTCAGACCGCATCAACAT
CATTCAACTGGAAAAGAAAGCTCAGGCATTAAAAATAGCCACATTAGCCTCCATAGAAGTACCAGAAGACATAGTACAAG
AAGGACAAATAGCCGACCCGCCAGGGATGGCAGAACTGATACGTTCAGTCCTCGCCGAAAACAAAATCAAAGCCAAGCAC
GTAGCCACAGCCATTCCCGGACGGGAAGCCGTGATTCGGTTAATTCCAGTCCCATCAGAACTCAACAACGAAGAACTGCG
AGAATACATGAATTCAGAAGCAGGGCTATACTTGCCATTTCCCAGAGAAGATGCCGACGTAGACTATCAAAAAATCGGCC
TATTTGTCGATGACGACGGCGTAGAAAAAGTGCAAGTTGTACTCGTAGCAACCCGCAAAGAAGTGACAGACACCTATCTA
GAAACCTTCAAAGAAGCCGGACTGATTATAGACGTGCTAGAAGTAACCAGCTTTGCCCTGATCCGCACCCTCAAAGACCA
ACTGCAACAATTTTCCTCCCAAGAGGCAGCAGTCTTGACAGATATAGAATTTGACAGCACCGAGTTAGCTATAGTAGTAG
ACGGCATACCACAATTCAACCGTACCATCCCGATCGGCACTTACCAAATCCAGACTGCCCTCAACGAAGCCATGAATCTG
CCACCAACCAGAGACACCAGCGAACTACAAGGCATGACCTTACCAGTAACAGATACAATGGGGACATCAGGGGATGCAAA
TCCGGGTACAAATGCGATGATTAAAGTCTTGGGAGAACTCGCAGACGAACTGCGTCGTTCAGTAGACTTTTATCTCAACC
AGAGCGAAGAACTAGAAGTAGCACAACTCCTGTTAGCAGGGCCAGGAGCTGCGATCGGCAAACTAGATGAATTTTTCATG
CAAAGATTAAGCTTGCCAGCTTCTCAAGTAGATCCCATCGACACCCTAGGACTCGAAGTCAGCGAAGAAATTAGCCCAGA
ACAGCGAGCAGGTTTAGGAGTAGCTTTAGGTTTAGGACTAAGGGAGGTTTTGTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-362)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

57.923

100

0.582