Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   RKE30_RS28205 Genome accession   NZ_CP134875
Coordinates   6519163..6520254 (-) Length   363 a.a.
NCBI ID   WP_313747119.1    Uniprot ID   -
Organism   Streptomyces sp. Li-HN-5-11     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 6514163..6525254
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RKE30_RS28185 (RKE30_28185) - 6514910..6515458 (-) 549 WP_313747115.1 adenine phosphoribosyltransferase -
  RKE30_RS28190 (RKE30_28190) secF 6515455..6516573 (-) 1119 WP_313747116.1 protein translocase subunit SecF -
  RKE30_RS28195 (RKE30_28195) secD 6516575..6518362 (-) 1788 WP_313747117.1 protein translocase subunit SecD -
  RKE30_RS28200 (RKE30_28200) yajC 6518494..6519000 (-) 507 WP_313747118.1 preprotein translocase subunit YajC -
  RKE30_RS28205 (RKE30_28205) ruvB 6519163..6520254 (-) 1092 WP_313747119.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  RKE30_RS28210 (RKE30_28210) ruvA 6520280..6520885 (-) 606 WP_313747120.1 Holliday junction branch migration protein RuvA -
  RKE30_RS28215 (RKE30_28215) ruvC 6520882..6521421 (-) 540 WP_313747121.1 crossover junction endodeoxyribonuclease RuvC -
  RKE30_RS28220 (RKE30_28220) - 6521545..6522297 (-) 753 WP_313747122.1 YebC/PmpR family DNA-binding transcriptional regulator -
  RKE30_RS28225 (RKE30_28225) pdxT 6522376..6522981 (-) 606 WP_313747123.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
  RKE30_RS28230 (RKE30_28230) pdxS 6523023..6523937 (-) 915 WP_399134220.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
  RKE30_RS28235 (RKE30_28235) - 6524082..6524696 (-) 615 WP_313747124.1 hypothetical protein -

Sequence

Protein


Download         Length: 363 a.a.        Molecular weight: 38883.30 Da        Isoelectric Point: 4.7885

>NTDB_id=808252 RKE30_RS28205 WP_313747119.1 6519163..6520254(-) (ruvB) [Streptomyces sp. Li-HN-5-11]
MNWDDTTDDAAERPDGPAGERLVGSVADREDQAVEAALRPKDLGEFIGQEKVREQLDLVLRAARARGATADHVLLSGAPG
LGKTTLSMIIAAEMGAPIRITSGPAIQHAGDLAAILSSLQEGEVLFLDEIHRMSRPAEEMLYMAMEDFRVDVIVGKGPGA
TAIPLELPPFTLVGATTRAGLLPPPLRDRFGFTAHMEFYEPAELERVVHRSAHLLDVEIEADGAAEIAGRSRGTPRIANR
LLRRVRDYAQVKADGIITREIAAAALAVYEVDARGLDRLDRAVLQALLKLFGGGPVGLSTLAVAVGEERETVEEVAEPFL
VREGLLARTPRGRIATPAAWEHLGLTPPRTSAAGNGQQDLFGA

Nucleotide


Download         Length: 1092 bp        

>NTDB_id=808252 RKE30_RS28205 WP_313747119.1 6519163..6520254(-) (ruvB) [Streptomyces sp. Li-HN-5-11]
ATGAACTGGGACGACACGACCGACGACGCCGCCGAGCGGCCCGACGGCCCCGCCGGGGAGCGGCTGGTGGGCTCTGTCGC
CGACCGTGAGGACCAGGCCGTCGAGGCCGCCCTGCGCCCCAAGGACCTGGGCGAGTTCATCGGCCAGGAGAAGGTCCGCG
AGCAGCTCGACCTCGTACTGCGCGCCGCGCGCGCGCGTGGGGCGACCGCCGACCACGTCCTGCTGTCCGGCGCCCCCGGC
CTGGGCAAGACCACCCTGTCGATGATCATCGCCGCCGAGATGGGCGCGCCGATCCGCATCACCAGCGGCCCGGCGATCCA
GCACGCCGGCGACCTCGCCGCGATCCTCTCCTCCCTCCAGGAGGGCGAGGTCCTCTTCCTCGACGAGATCCACCGCATGT
CCCGGCCCGCCGAGGAGATGCTCTACATGGCCATGGAGGACTTCCGCGTCGACGTCATCGTCGGCAAGGGCCCCGGCGCC
ACCGCCATCCCGCTCGAACTGCCGCCGTTCACCCTGGTCGGCGCCACCACGCGCGCGGGCCTGCTGCCGCCGCCGCTGCG
CGACCGCTTCGGCTTCACCGCGCACATGGAGTTCTACGAGCCCGCCGAACTGGAGCGGGTCGTCCATCGCTCGGCGCACC
TGCTCGACGTGGAGATCGAGGCCGACGGCGCCGCCGAGATCGCCGGCCGCTCCCGCGGCACGCCCCGCATCGCCAACCGC
CTGCTGCGCCGCGTACGCGACTACGCGCAGGTCAAGGCCGACGGGATCATCACCCGCGAGATCGCCGCGGCGGCCCTCGC
CGTCTACGAGGTCGACGCCCGCGGCCTCGACCGGCTCGACCGGGCGGTCCTGCAGGCGCTGCTGAAGCTGTTCGGCGGCG
GTCCCGTCGGTCTGTCCACGCTCGCCGTCGCGGTGGGGGAGGAGCGCGAGACCGTCGAGGAGGTCGCCGAGCCGTTCCTC
GTCCGTGAGGGCCTGCTCGCCCGCACCCCGCGCGGACGTATCGCCACCCCCGCGGCCTGGGAGCACCTCGGTCTGACCCC
GCCGCGCACGTCAGCCGCGGGAAACGGACAACAGGACTTGTTCGGGGCGTGA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(274-343)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(38-196)

RuvB AAA lid domain (AAA_lid_4)

(199-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

55.455

90.909

0.504
  ruvB Synechocystis sp. PCC 6803

54.777

86.501

0.474
  ruvB Streptococcus pneumoniae TIGR4

53.333

86.777

0.463
  ruvB Streptococcus pneumoniae R6

53.333

86.777

0.463
  ruvB Streptococcus pneumoniae D39

53.333

86.777

0.463
  ruvB Helicobacter pylori 26695

50

89.807

0.449