Detailed information NTG0808021

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P4B20_RS02075
• Genome accession: NZ_CP120706
• Coordinates: 447738..448886 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain NY11104
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 442738..453886

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P4B20_RS02050 (P4B20_02050)	-	443889..444185 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
P4B20_RS02055 (P4B20_02055)	-	444182..444775 (-)	594	WP_003084544.1	YggT family protein	-
P4B20_RS02060 (P4B20_02060)	proC	444786..445607 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
P4B20_RS02065 (P4B20_02065)	-	445619..446311 (-)	693	WP_003118803.1	YggS family pyridoxal phosphate-dependent enzyme	-
P4B20_RS02070 (P4B20_02070)	pilT	446526..447560 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
P4B20_RS02075 (P4B20_02075)	pilU	447738..448886 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
P4B20_RS02080 (P4B20_02080)	czcD	448894..449793 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
P4B20_RS02085 (P4B20_02085)	-	449948..450352 (+)	405	WP_003084560.1	NINE protein	-
P4B20_RS02090 (P4B20_02090)	-	450595..451968 (+)	1374	WP_179063077.1	pyridoxal-phosphate dependent enzyme	-
P4B20_RS02095 (P4B20_02095)	-	451965..453149 (+)	1185	WP_003117897.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=808021 P4B20_RS02075 WP_003100959.1 447738..448886(+) (pilU) [Pseudomonas aeruginosa strain NY11104]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=808021 P4B20_RS02075 WP_003100959.1 447738..448886(+) (pilU) [Pseudomonas aeruginosa strain NY11104]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382