Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   RM370_RS10705 Genome accession   NZ_CP134716
Coordinates   2262461..2263471 (+) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli strain RIVM_C040348     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2257461..2268471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RM370_RS10675 (RM370_10675) nudB 2259182..2259634 (+) 453 WP_077393227.1 dihydroneopterin triphosphate diphosphatase -
  RM370_RS10680 (RM370_10680) yebC 2259663..2260403 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  RM370_RS10685 (RM370_10685) ruvC 2260438..2260959 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  RM370_RS10690 (RM370_10690) yebB 2260961..2261563 (-) 603 WP_001024942.1 YebB family permuted papain-like enzyme -
  RM370_RS10695 (RM370_10695) yobI 2261637..2261702 (+) 66 WP_072123646.1 stress response small protein YobI -
  RM370_RS10700 (RM370_10700) ruvA 2261841..2262452 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  RM370_RS10705 (RM370_10705) ruvB 2262461..2263471 (+) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  RM370_RS10710 (RM370_10710) znuB 2263619..2264404 (-) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  RM370_RS10715 (RM370_10715) znuC 2264401..2265156 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  RM370_RS10720 (RM370_10720) znuA 2265235..2266167 (+) 933 WP_001339711.1 zinc ABC transporter substrate-binding protein ZnuA -
  RM370_RS10725 (RM370_10725) mepM 2266183..2267505 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=807625 RM370_RS10705 WP_000568522.1 2262461..2263471(+) (ruvB) [Escherichia coli strain RIVM_C040348]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=807625 RM370_RS10705 WP_000568522.1 2262461..2263471(+) (ruvB) [Escherichia coli strain RIVM_C040348]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAATATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCAATGCTCACTAACCTTGAACCACA
CGATGTACTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGTGCGCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494